Cell-Penetrating Peptides

The modern reference on the rational design and development of cell-penetrating peptides (CPPs) for use in cellular delivery of drugs and various other cargoes.

Autorentext
Prof. Makoto Oba received his Ph.D. degree in pharmaceutical sciences from Kyushu University in 2004. He joined the faculty of the University of Tokyo (2004-2010) and Nagasaki University (2010-2020). He moved to Kyoto Prefectural University of Medicine on 2020 as full Professor at Graduate School of Medicine. He has authored over 100 scientific publications and has received several awards, including the Pharmaceutical Society of Japan Award for Young Scientists (2013), Nagasaki Prefectural Science and Technology Award for Young Scientists (2016), and the Incentive Award in Synthetic Organic Chemistry (2019).

Dr. Yosuke Demizu received his Ph.D. in pharmaceutical sciences from Kyushu University in 2006. He joined the faculty of Tokushima Bunri University (2004-2005) and then at Nagasaki University (2006-2008). He moved to the National Institute of Health Sciences (NIHS) as a researcher, and was promoted to head the division of organic chemistry in 2017. He has authored over 150 scientific publications and has received several awards, including the Incentive Award in Synthetic Organic Chemistry, Japan (2006, 2010) and the Award for Young Investigators from the Japanese Peptide Society (2014).

Klappentext

The definitive reference on the rational design of cell-penetrating peptides enables readers to develop tailor-made peptides for their specific needs. In recent years, cell-penetrating peptides (CPPs) have become valuable tools for the cellular delivery of proteins, nucleic acids, and drugs. These small peptide sequences can be artificially designed and synthesized with custom-made characteristics to mediate the efficient and non-toxic transport of biomolecules, drugs, or nanoparticles into the cell. Cell-Penetrating Peptides: Design, Development, and Applications provides an up-to-date account of the development and use of CPPs for delivering membrane-impermeable bioactive molecules into cells. Bringing together contributions from leading researchers from around the world, this comprehensive volume describes the characteristics and mechanisms of CPPs as well as their application in both medicine, biotechnology and agriculture.

• Covers rational design and development of cell-penetrating peptides for use in cellular delivery of small molecule drugs, proteins, nucleic acids, and nanoparticles
• Presents the chemical and biological characteristics of CPP action in vitro and in vivo
• Describes the structure and design principles of both synthetic and naturally occurring CPPs

• Discusses key medical applications of CPPs such as oral delivery, intranasal delivery, and clinical trials Cell-Penetrating Peptides: Design, Development, and Applications is an essential resource for biochemists, medicinal chemists, molecular biologists, biotechnologists, and researchers studying CPPs in both academia and industry.

  Inhalt
  Introduction

  PART 1: DESGIN OF CPPS
  Classification of cell-penetrating peptides
  Arginine-rich cell-penetrating peptides: recent advances of design and applications for intracellular delivery
  Cationic cell-penetrating peptides
  Amphipathic cell-penetrating peptides
  Hydrophobic cell-penetrating peptides
  Foldamers

  PART 2: MECHANISM OF CPPS
  Peptide Structure: Electrophysiological analysis, nuclear magnetic resonance analysis, and molecular dynamics simulation of direct penetration of cell-penetrating peptides through bilayer lipid membranes
  Cellular uptake mechanisms of arginine-rich cell-penetrating peptides
  Endosomal escape
  Pharmacokinetics of therapeutic and diagnostic agents conjugated with cell-penetrating peptides

  PART 3: DELIVERY TOOLS
  Drug delivery
  Peptide & protein delivery
  Cell-penetrating peptides for nucleic acid delivery
  PPMOs: a case study for cell-penetrating peptide application
  Cell penetrating peptide-conjugated polymer micelles for pDNA/siRNA delivery
  Lipid-based nanoparticles using cell-penetrating peptides

  PART 4: APPLICATIONS
  Cell-penetrating peptides as tools to facilitate oral delivery of biopharmaceuticals
  Intranasal delivery
  Clinical trials
  Applications in plants