Chromosome Analysis Protocols

Chromosomes, as the genetic vehicles, provide the basic material for a large proportion of genetic investigations, from the construction of gene maps and models of chromosome organization, to the inves tigation of gene function and dysfunction. The study of chromosomes has developed in parallel with other aspects of molecular genetics, beginning with the first preparations of chromosomes from animal cells, through the development of banding techniques, which permitted the unequivocal identification of each chromosome in a karyotype, to the present analytical methods of molecular cytogenetics. Although some of these techniques have been in use for many years, and can be learned relatively easily, most published scientific reportsas a result of pressure on space from editors, and the response to that pressure by authorscontain little in the way of technical detail, and thus are rarely adequate for a researcher hoping to find all the necessary information to embark on a method from scratch. A new user needs not only a detailed description of the methods, but also some help with problem solving and sorting out the difficulties en countered in handling any biological system. This was the require ment to which the series Methods in Molecular Biology is addressed, and Chromosome Analysis Protocols forms a part of this series.

Zusammenfassung
...a very worthwhile book...invaluable for those trying to venture into the area of molecular cytogenetics...Provides a ready source of both old and new cytogenetic methods and contains a wealth of troubleshooting tips from experienced workers in the field.-Modern Pathology

Inhalt
Mitotic Metaphase Chromosome Preparation from Peripheral Blood for High Resolution.- Chromosome Preparation from Hematological Malignancies.- Meiotic Chromosome Preparation.- Preparation of Chromosomes for Scanning Electron Microscopy.- Immortalized Cell Lines.- Chromosome Banding and Identification Absorption Staining.- Chromosome Banding and Identification.- Chromosome Banding.- Inhibition of Chromosome Condensation.- Analysis of Chromosomes with Restriction Endonucleases and DNase Hypersensitivity.- Automatic Karyotype Analysis.- Bivariate Chromosome Analysis Using a Commercial Flow Cytometer.- Chromosome Sorting by Flow Cytometry.- Electrophoretic Karyotype Analysis Pulsed Field Gel Electrophoresis.- Immunofluorescence Techniques Applied to Mitotic Chromosome Preparations.- Immunocytochemical Techniques Applied to Meiotic Chromosomes.- Immortalized Cell Lines.- Oligonucleotide Primed in Situ DNA Synthesis (PRINS).- Electron Microscopic Localization of in Situ Hybrids.- Protocols for Chromosome-Mediated Gene Transfer.- Construction of Chromosome-Specific Libraries of Yeast Artificial Chromosome Recombinants from Somatic Hybrid Cell Lines.- Yeast Artificial Chromosome Recombinants in a Global Strategy for Chromosome Mapping.- Chromosome Dissection and Cloning.- Molecular Analysis of Chromosome Aberrations in Hematological Malignancies.- Molecular Analysis of Chromosome Aberrations.- Alu- and LI-Primed PCR-Generated Probes for Nonisotopie in Situ Hybridization.- Chromosome Substructure Investigation.