Effect of cryopreservation on sperm subpopulation in bovine

A common feature of mammalian ejaculates is the heterogeneity of sperm motility and morphology, and of almost all sperm functions, seen in both fertile and subfertile individuals. Such heterogeneity of sperm motility is reflected in the existence of discrete sperm subpopulations (SP) that differ in their kinematic values. Sperm subpopulations with different motility characteristics were first determined by multivariate cluster analysis of CASA-derived kinematic parameters of human epididymal sperm (Davies et al., 1993). After that, several research groups identified the existence of motile sperm subpopulations in ejaculates of many different mammalian species: marmoset (Holt, 1996), gazelles and boars (Abaigar et al., 1999), stallions (Quintero-Moreno et al., 2003), dogs (Nuñez Martínez et al., 2006a, 2006b), bulls (Muiño et al., 2008a), etc. In bovine ejaculates, the sperm subpopulation with the highest velocity and progressiveness, but not the total percentage of motile sperm, was significantly correlated with sperm cryosurvival (Muiño et al. 2008a; 2009b) and with the outcomes of ZBA and IVF tests (Ferraz et al., 2014).

Autorentext

Dr. Rodrigo Muiño Otero promovierte 2008 in Biotechnologie der Fortpflanzung. In den letzten zwanzig Jahren hat er in der Medizin und Reproduktion von Milchkühen gearbeitet. Seine Forschungsinteressen liegen in den Bereichen Biotechnologie und Metabolismus bei Rindern. Er ist Autor mehrerer Artikel und Bücher, die in Fachzeitschriften veröffentlicht wurden.