Intracellular Thermometry with Fluorescent Molecular Thermometers

Written for an audience of researchers and professionals in the bioanalytical sciences, this comprehensive overview on intracellular temperature sensing describes fundamentals, applications and future perspectives of this promising new technology.

Autorentext

Seiichi Uchiyama has been an Assistant Professor at the University of Tokyo since 2005. After receiving his academic degrees in Pharmacy from the University of Tokyo with Prof. Kazuhiro Imai he spent three years as a postdoctoral researcher at Nara Women's University with Prof. Kaoru Iwai and at Queen's University of Belfast with Prof. A. Prasanna de Silva. After returning to the University of Tokyo in 2005, he started his career as an experimental researcher and continues to work as an independent leader of scientific projects funded by the Japanese government. His scientific interests include analytical and photophysical chemistry and the development of fluorescent sensors based on novel functional mechanisms.

Klappentext

Understand a vital new bioanalytical technique with this comprehensive introduction to measuring temperature on the cellular scale Most organisms have highly controlled body temperatures, fluctuations in which are therefore sensitive indicators of changes in body function. In recent years, the development of fluorescent molecular thermometers and related intracellular temperature probes has enabled researchers to track these fluctuations at the cellular rather than the organismic level, opening up a whole new field of study in cell and molecular biology. Intracellular Thermometry with Fluorescent Molecular Thermometers provides bioanalytical researchers with an introduction to these technologies and their current and future applications. Starting off with a discussion of temperature as a key factor in biological regulation, it provides an authoritative overview of available fluorescent temperature probes, their characteristics and potential applications. Intracellular Thermometry with Fluorescent Molecular Thermometers readers will also find:

• Step by step instructions for constructing an intracellular thermometry experiment and validating results
• Comprehensive discussion of existing applications

• A vision for the future development of thermal biology as an independent discipline Authored by a pioneer in the field of intracellular thermometry, Intracellular Thermometry with Fluorescent Molecular Thermometers is ideal for researchers in analytical chemistry, cell biology, molecular biology, biophysics, or any related subjects.

  Inhalt
  1 Temperature in Biological Species
  1.1 Temperature in Our Life
  1.2 Body Temperature
  1.3 Effects of Environmental Temperature on Biological Function
  1.4 Molecules Associated with Biological Responses to Environmental Temperature

  2 Development of Fluorescent Molecular Thermometers
  2.1 Physical Chemistry of Fluorescence
  2.2 Fluorescent Molecular Thermometers
  2.2.1 Small Organic compounds involving intersystem crossing
  2.2.2 Small Organic Compounds Involving Conformational Change
  2.2.2 Organometallic Complexes
  2.2.3 Excimers and Exciplex
  2.2.4 Host-Guest Complexes
  2.2.5 Thermoresponsive Macromolecules
  2.2.6 Inorganic Materials
  2.2.7 Others

  3 Intracellular Thermometry with Fluorescent Molecular Thermometers
  3.1 Early Attempts for Cellular Thermometry
  3.2 Introduction of Fluorescent Molecular Thermometers into a Living Cell
  3.3 Cytotoxicity Assessment
  3.4 Practice of Intracellular Thermometry with Fluorescent Molecular Thermometers
  3.5 Intracellular Thermometry with Organelle-targeted Fluorescent Molecular Thermometers
  3.6 Intracellular Thermometry of Brown Adipocytes
  3.7 Application of Intracellular Thermometry in Various Biological Fields

  4 Cellular Thermometry Based on Non-fluorometric Principles
  4.1 Infrared Thermometry
  4.2 Photoacoustic Thermometry
  4.3 Raman Thermometry
  4.4 Use of Transmission Spectroscopy
  4.5 Thermocouples
  4.6 Resonant Thermal Sensors
  4.7 Bimaterial Microcantilevers
  4.8 Thermistors

  5 Reliability of Intracellular Thermometry with Fluorescent Molecular Thermometers
  5.1 Validity of a Calibration Curve
  5.2 Possible Artifacts in Thermometry of Biological Samples
  5.3 Difference between Experimentally Observed and Theoretically Calculated Intracellular Temperature Variation

  6 The Future of Intracellular Thermometry
  6.1 In vivo Thermometry
  6.2 Thermal Medicine
  6.3 Thermal Signaling
  6.4 Absolute Determination of Intracellular Temperature
  6.5 Simultaneous Measurement of Multiple Parameters
  6.6 Intracellular Thermometry of Plant Cells

  Appendix
  A.1 Review and account items on fluorescent molecular thermometers
  A.2 Comprehensive collection of fluorescent polymeric thermometers based on the combination of a thermoresponsive polymer and an environment-sensitive fluorophore
  A.3 Comprehensive collection of fluorescent nanogel thermometers based on the combination of a thermoresponsive polymer and an environment-sensitive fluorophore
  A.4 Non-covalent mixtures of a thermoresponsive polymer and an environment-sensitive fluorophore
  A.5 Fluorescent logic gates based on the combination of a thermoresponsive polymer and an environment-sensitive fluorophore
  A.6 Fluorescent inorganic thermometers