Methods in Modern Biophysics

Incorporating recent dramatic advances, this textbook presents a fresh and timely introduction to modern biophysical methods. It presents novel information, and is the first book to survey and explain the ten key biophysical methods.

Presents novel information which cannot be found in other textbooks and monographs Provides one of the most up to date presentations of current biophysics Only book which covers all of the 10 currently most important biophysical methods Details exciting recent discoveries and developments in the fields of biophysical nanotechnology, scanning probe microscopy, ion mobility spectrometry, mass spectrometry and proteomics Introduces rapid partial protein ladder sequencing - an important method for fast and highly sensitive identification of proteins Describes the determination of interaction surfaces in protein complexes by mass spectrometric mapping of binding of chemical linkers - a method to support the high-resolution structural analysis of large macromolecular assemblies Well above 250 figures support a quick understanding of technological details plus worked examples in the text and solutions to problems facilitate teaching Includes supplementary material: sn.pub/extras

Inhalt
The three-dimensional structure of proteins.- Liquid chromatography of biomolecules.- Mass spectrometry.- X-ray structural analysis.- Protein infrared spectroscopy.- Electron microscopy.- Scanning probe microscopy.- Biophysical nanotechnology.- Proteomics: high throughput protein functional analysis.- Ion mobility spectrometry.- -Value analysis.- Evolutionary computer programming.- Rapid partial protein ladder sequencing.- Surface labeling analysis of protein interactions.- Conclusions.- References.