Murine Interferon Regulatory Factor-2 (IRF-2) and Escherichia coli

Interferon Regulatory Factor-2 is a vertebrate transcription factor having oncogenic nature; it antagonises Interferon Regulatory Factor-1, a tumor suppressor gene, functions. Knockout mice for IRF-2 gene showed defect in B-cell lymphopoeisis, they died following LCMV (Lymphocytic Choriomeningitis Virus) infections. IRF-2 is functionally just opposite of IRF-1. Interestingly, one would have thought that as IRF-1-/- (knock-out) mice showed lack of CD8+T cells and low cytotoxicity of NK cells, the over production of IRF-2 in mice may restore it. But it does not happen so, the reason being unknown. However, IRF-2 binds at Interferon Stimulated Response Element sequence in viral infected cell. This book is about mouse IRF-2 gene cDNA cloning and expression of chimeric protein in E.coli. This study will serve as a platform for further functional analysis of chimeric IRF-2, which is potent oncogene in aberrant conditions.

Autorentext

Prakash K. est professeur adjoint au département de biotechnologie de l'université centrale de South Bihar, à Patna. Il a obtenu son doctorat en biologie moléculaire à la JNU de New Delhi. Auparavant, il a travaillé comme chercheur post-doctoral pendant plusieurs années dans différents instituts de recherche réputés tels que l'ICGEB et le NII, New Delhi.