Nucleic Acid Electrophoresis

Electrophoresis is a powerful method to analyze nucleic acids (DNA, RNA). Various sophisticated techniques such as capillary electrophoresis, pulsed-field electrophoresis, fingerprinting using RFLP and RAPD, DNA sequencing, and mobility shift assay are described in detail. The required apparatus, appropriate use, preparation of probes, gel staining, interpretation of results, tricks for troubleshooting, manufacturers addresses, helpful Internet resources as well as specific applications, e.g. in legal medicine, microbiology and agriculture, are presented by leading experts.

Step-by-step protocols with materials, troubleshooting and suppliers sections for immediate use at the lab bench All technical details needed to perform successful experiments are included Clear structure and presentation Protocols are written by experts in the relevant techniques

Inhalt
1 DNA Gel Electrophoresis.- 2 Denaturating Electrophoresis in DNA Sequencing: A Brief History and Current Protocols.- 3 An Introduction to DNA Fingerprinting Using RFLP and RAPD Techniques.- 4 Pulsed-Field Gel Electrophoresis for Genomic Fingerprinting of Pathogenic Bacteria.- 5 Ultrasensitive Silver Based Stains for Nucleic Acid Detection.- 6 The Analysis of Microsatellites by Silver Staining of Nondenaturing Polyacrylamide Gels.- 7 Capillary Electrophoretic Separation of DNA Fragments.- 8 Rapid Detection of Hepatitis C Virus in Plasma and Liver Biopsies by Capillary Electrophoresis.- 9 Electrophoresis in the Field of Forensic Science.- 10 The Electrophoretic Mobility Shift Assay (EMSA) for Detection and Analysis of Protein-DNA Interactions.- 11 Mobility Shift Analysis of Protein-DNA Complexes by Capillary Electrophoresis.- 12 Polyacrylamide Gel Electrophoresis of DNA/Protein Complexes.- 13 Detection of DNA Curvature Using Transverse Pore Gradient Polyacrylamide Gel Electrophoresis.