Polypeptide Materials

This book details the synthesis and assembly of polypeptide materials across length scales, i.e. proteins and peptides, their precursors, conjugates, and derivatives. A particular emphasis is made on measurement tools and procedures for material characterization, both physicochemical and functional. Written for the highly successful Methods in Molecular Biology series, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.
Authoritative and practical, Polypeptide Materials: Methods and Protocols serves to reflect the inter-disciplinary nature of molecular biology as well as the importance of developing innovative measurement methods to advance this vital area of research.

Includes cutting-edge techniques Provides step-by-step details essential for reproducible results Contains key implementation advice from the experts

Inhalt

A Shortcut to the Synthesis of Peptide Thioesters Using an HF-Free Approach.- SnoopLigase-Mediated Peptide-Peptide Conjugation and Purification.- Peptide Nanoparticles for Gene Packaging and Intracellular Delivery.- Synthesis and Application of Peptide-siRNA Nanoparticles from Disulfide-Constrained Cyclic Amphipathic Peptides for the Functional Delivery of Therapeutic Oligonucleotides to the Lung.- FRET-Mediated Observation of Protein-Triggered Conformational Changes in DNA Nanostructures.- Molecular Simulations Guidelines for Biological Nanomaterials: From Peptides to Membranes.- Functional Peptide Nanocapsules Self-Assembled from b-Annulus Peptides.- Electrostatic Self-Assembly of Protein Cage Arrays.- Design and Generation of Self-Assembling Peptide Virus-Like Particles with Intrinsic GPCR Inhibitory Activity.- Imaging and 3D Reconstruction of De Novo Peptide Capsids.- Monitoring the Assembly and Aggregation of Polypeptide Materials by Time-Resolved Emission Spectra.- Forming Low Molecular Weight Hydrogels by Electrochemical Methods.- In Situ Measurements of Polypeptide Samples by Dynamic Light Scattering: Membrane Proteins, a Case Study.- Measurement of Peptide Coating Thickness and Chemical Composition Using XPS.- Imaging the Effects of Peptide Materials on Phospholipid Membranes by Atomic Force Microscopy.- Microfluidic Single-Cell Phenotyping of the Activity of Peptide-Based Antimicrobials.- Ultramicrotomy Analysis of Peptide-Treated Cells.