Selectively Inhibiting Ras-p21 by a Synthetic SOS Plasmid

Scientific work over the past 30 years revealed thatSOS protein is directly involved in the activationof ras-p21.The aim of this research project was tosynthesize a plasmid which would ultimately expressa SOS peptide, 994-1004, which was proven to inhibitthe Val 12 ras-p21 pathway in Xenopus laevisoocytes. The SOS plasmid consisted of a vector,pOPRSVI/MCS, ligated to DNA oligonucleotidescorresponding to the SOS peptide of interest.Different SOS plasmid concentrations, ranging from1ug/uL to 5.138ug/uL, were either co-microinjectedwith Val 12 p21 or microinjected into oocytesfollowed by incubation in insulin or inprogesterone. At the 48th hour time point, theresults showed that the 3.225ug/uL SOS plasmid gavethe most inhibition on the oncogenic pathway whilethe wild-type p21 pathway was unaffected by thisconcentration. This data suggest that the 3.225ug/uL SOS plasmid is a specific inhibitor of theoncogenic ras-p21 pathway.

Autorentext

Ethia E Vitalis
Brooklyn, NY 11215
eevitalis04@gmail.com

Ambitious Molecular Biology Grad student with strong background
and education in the principles applied to science and its
techniques

Klappentext

Scientific work over the past 30 years revealed that
SOS protein is directly involved in the activation
of ras-p21.The aim of this research project was to
synthesize a plasmid which would ultimately express
a SOS peptide, 994-1004, which was proven to inhibit
the Val 12 ras-p21 pathway in Xenopus laevis
oocytes. The SOS plasmid consisted of a vector,
pOPRSVI/MCS, ligated to DNA oligonucleotides
corresponding to the SOS peptide of interest.
Different SOS plasmid concentrations, ranging from
1ug/uL to 5.138ug/uL, were either co-microinjected
with Val 12 p21 or microinjected into oocytes
followed by incubation in insulin or in
progesterone. At the 48th hour time point, the
results showed that the 3.225ug/uL SOS plasmid gave
the most inhibition on the oncogenic pathway while
the wild-type p21 pathway was unaffected by this
concentration. This data suggest that the 3.225
ug/uL SOS plasmid is a specific inhibitor of the
oncogenic ras-p21 pathway.