'stx 1' and 'stx 2' toxigenic genes in Escherichia coli isolates from local poultry droppings

PCR detection of 'stx 1' and 'stx 2' toxigenic genes in multiple antibiotic resistant Escherichia coli population and phenotypic detection of ESBL producing Escherichia coli isolated from traditional local variety of poultry revealed that out of 30 samples collected 20 were positive for E.coli. All the 20 isolates were subjected for the detection of 2 toxin genes (stx1 & stx2 genes) by PCR. Only 3 isolates (L3, L4 & L5) gave rise to PCR product against its specific primers of stx1. All the isolates were negative for the presence of stx2 gene. Thus 15% of E.coli population under this study is potential isolates and indicates that healthy retail poultry birds are the carrier of E.coli pathogenic strains. The poultry birds may therefore, be considered as a major reservoir of E.coli.

Autorentext

La Dra. Dipanjali Saikia trabaja actualmente como profesora adjunta en el Departamento de Biotecnología del Kaliabor College, Kuwaritol (Assam): INDIA. Se doctoró en la Universidad de Gauhati. Ha superado los exámenes GATE y SLET. Dirigió varios proyectos sobre plantas de la región del NE a nivel de grado. Ha publicado varios artículos en revistas de renombre, actas y capítulos de libros.

Klappentext

PCR detection of 'stx 1' and 'stx 2' toxigenic genes in multiple antibiotic resistant Escherichia coli population and phenotypic detection of ESBL producing Escherichia coli isolated from traditional local variety of poultry revealed that out of 30 samples collected 20 were positive for E.coli. All the 20 isolates were subjected for the detection of 2 toxin genes (stx1 & stx2 genes) by PCR. Only 3 isolates (L3, L4 & L5) gave rise to PCR product against its specific primers of stx1. All the isolates were negative for the presence of stx2 gene. Thus 15% of E.coli population under this study is potential isolates and indicates that healthy retail poultry birds are the carrier of E.coli pathogenic strains. The poultry birds may therefore, be considered as a major reservoir of E.coli.