Methionine Oxidation in Human Prion Protein

The aim of this thesis was to examine the pathological relevance of the oxidation state of methionine (Met) side chains in neurodegenerative disorders such as sporadic prion disease. First for better understanding the oxidative event, the effect and consequences of Met oxidation in the recombinant human cellular prion protein (rhPrPC23-231) were studied. Second the incorporation of stable Met analogs, norleucine and methoxinine, in rhPrPC23-231 -mimicking the reduced and oxidized state of Met, respectively - was performed. The results strongly indicate a correlation of the secondary structure conversion in rhPrPC with the oxidative state of the Met residues. In the future, this approach will certainly be useful for studying diseases, which arise from protein misfolding due to oxidative stress.

Autorentext

Dr. Christina Wolschner studied Food- and Biotechnology at theUniversity of Natural Resources and Applied Life Sciences inVienna, Austria. During her studies, she stayed one year at theLa Trobe University in Melbourne, Australia. In May 2009 shesuccessfully finished her Phd at the Max-Planck-Institute ofBiochemistry in Martinsried, Germany.