Bacterial Persistence

This volume presents a collection of methods that have contributed to the current understanding of bacterial persisters. Chapters in the book detail general guidelines for measuring persister levels in bacterial *cultures, strategies to enrich and resuscitate persister subpopulations, single-cell approaches for visualizing and characterizing persisters, omics techniques and cellular and animal models for studying persistence. Written in the highly successful Methods in Molecular Biology* series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.

Authoritative and cutting-edge, Bacterial Persistence: Methods and Protocols, Second Edition aims to be a useful practical guide to researchers to help further their study in this field.

Includes cutting-edge methods and protocol Provides step-by-step detail essential for reproducible result

Inhalt
Studying Bacterial Persistence: Established Methods And Current Advance.- Antibiotic Tolerance and Persistence Studied Throughout Bacterial Growth Phases.- A Robust Method For Generating, Quantifying, and Testing Large Numbers of Escherichia coli persisters.- Enrichment of Persister Cells through -lactam-induced Filamentation and Size Separation.- Methods for Enrichment of Bacterial Persister Populations For Phenotypic Screens And Genomic Studies.- Observing Bacterial Persistence at Single-Cell Resolution.- Phenotypic Characterization of Antibiotic Persisters at the Single-Cell Level: From Data Acquisition to Data Analysis.- Microfluidics for Single-Cell Study of Antibiotic Tolerance and Persistence Induced by Nutrient Limitation.- Counting Chromosomes in Individual Bacteria to Quantify their Impacts on Persistence.- Analyzing PERSISTER PROTEOMES with SILAC and Label-Free Methods.- Defining Proteomic Signatures To Predict Multi-Drug Persistence in Pseudomonas aeruginosa.- The use of Experimental Evolution to Study The Response of Pseudomonas aeruginosa to Single or Double Antibiotic Treatment.- Detecting persister awakening determinants.- Monitoring Persister Resuscitation with Flow Cytometry.- Stimulating Aminoglycoside Uptake to Kill Staphylococcus aureus persisters.- In vitro Models for the Study of the Intracellular Activity Of Antibiotics.- Analysis of Salmonella Persister Population Sizes, Dynamics Of Gut Luminal Seeding, And Plasmid Transfer In Mouse Models Of Salmonellosis.- Studying Antibiotic Persistence During Infection.

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