Immobilized Penicillinase

This study aims at evaluating the lowest cost immobilized penicillin G acylase (PGA) by using two natural treated polymers, ( -carrageenan and alginate) and comparing them with already present support in the market like Eupergit ®C, for obtainning 6 -aminopenicillanic acid (6-APA), which is considered as the raw material in production of semi-synthetic antibiotics as ampicillin and amoxicillin.PGA enzyme was selected for immobilization process as it hydrolyses penicillin G (PG) to produce 6-APA, which is the precursor of 65% of the worldwide antibiotics (e.g., ampicillin, pencillin K, pencillin F, amoxicillin). Therefore, the annual consumption of PGA is estimated to be in the range of 10-30 million tons. The enzymatic production of 6-APA has replaced the multi step chemical conversion and made it cheaper with an efficiency of ~80-90%. However, in drug industries, enzymes are preferred to be immobilized on solid supports rather than being used in the free state since they can be separated easily from the product. Moreover, they could be reused for tens of time, which saves time and money.

Autorentext
Osama Ali, AliAli Osama Ali Ahmed Hassan-M.Sc. in biochemistry-faculty of science-Helwan University, june 2013.-Premaster courses in biochemistry-faculty of science-Helwan University, may 2008.-B.Sc. of chemistry/biochemistry-faculty of science-Helwan University, may 2006. (graduation degree: Excellent with honours).Egypt.