Metastasis Research Protocols

Now in a second, revised edition, this practical companion to the methodologies of researching cancer growth includes topical introductions and stepwise, reproducible protocols for laboratory processes, as well as invaluable troubleshooting tips.

Diverse molecular, cellular, and environmental events must all come together to allow the successful formation of secondary cancers, metastases. The second edition of Metastasis Research Protocols, brings together the most up to date versions of the seminal techniques that were presented in the first edition and also includes new techniques that have recently been shown to be important in illuminating the processes underlying this important area of biology. Presented by top scientists, the collection includes a wide spectrum of articles encompassing important key methods and to introduce new methods which are making an impact in the area of metastasis research. Volume 1 includes key cellular and molecular techniques relevant to the exploration of cancer cells and tissues, the focus is on the tools that have been shown to be helpful in unravelling the molecular processes important in cancer metastasis. Written in the highly successful Methods in Molecular Biology™ series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and key tips on troubleshooting and avoiding known pitfalls.

Authoritative and practical, Metastasis Research Protocols, Second Edition seeks to aid scientists in the further study of new methods in the area of metastasis research.

Details key cellular and molecular techniques relevant to the exploration of cancer cells and tissues Provides step-by-step detail essentail for reproducible results Contains key notes and implementation advice from the experts Includes supplementary material: sn.pub/extras

Inhalt

Basic Immunocytochemistry for Light Microscopy.-Lectin Histochemistry to Detect Altered Glycosylation in Cells and Tissues.-Histopathological Assessment of Metastasis.-Immunocytochemical Detection and Characterisation of Individual Micrometastatic Tumour Cells.-The Polymerase Chain Reaction (PCR).-SDS-PAGE and Western Blotting Techniques.-2-DE Based Proteomics for the Analysis of Metastasis Associated Proteins.-Assessment of Gelatinases (MMP-2 and MMP-9) by Gelatin Zymography.-Determination of Cell Specific Receptor Binding using a Combination of Immunohistochemistry and in vitro Autoradiography: Relevance to Therapeutic Receptor Targeting in Cancer.-Fluorescence in Situ Hybridization (FISH) for Cancer Related Studies.-Using a Quartz Crystal Microbalance Biosensor for the Study of Metastasis Markers on Intact Cells.-Cell Separations by Flow Cytometry.-Detection of Putative Cancer Stem Cells of the Side Population Phenotype in Human Tumor Cell Cultures.-Genes Involved in the Metastatic Cascade of Medullary Thyroid Tumours.-High-resolution Quantitative Methylation Analysis of microRNA Genes using Pyrosequencing™.-RNAi Technology to Block the Expression of Molecules Relevant to Metastasis: The Cell Adhesion Molecule CEACAM1 as an Instructive Example.-Galectin-3-Binding and Metastasis.-Lectin Array Based Strategies for Identifying Metastasis-associated Changes in Glycosylation.