A Suicide Vector Design for Cells Expressing HIV-1 Regulatory Genes

CHF 78.70
Auf Lager
SKU
V2EU4SSVV7H
Stock 1 Verfügbar
Free Shipping Kostenloser Versand
Geliefert zwischen Mi., 08.10.2025 und Do., 09.10.2025

Details

In this study, expression profiles of the toxic gene
HSV-1 TK on parental HeLa cells and HeLa cells
expressing HIV-1 regulatory genes was desired to be
assessed. The toxic gene (suicide gene) presumed to
create the desired effect was placed under the
transcriptional control of HIV promoter LTR and so
that the expression of the toxic gene was made
dependent upon the tat regulator gene of HIV. In
order to prevent leaky gene expression stemming from
the basal gene expression from LTR even if it was not
induced by Tat, and thereby having potential to
damage healthy cells, the prerequisite cis-acting DNA
sequences were cloned downstream of the toxic gene.
So that, the transcripts produced could retain in the
nucleus and would require the function of a second
regulator protein Rev for being transmitted into
the cytoplasm. However, since the generation of
stable cell lines expressing Tat and Rev proteins
could not be achieved, the possibility of working
with the suicide vector constructed for this purpose
was out of question. In this book, the strategies and
the handicaps will be discussed for researchers who
aim to develop cloning based approaches to prevent
HIV infection.

Autorentext

Studied Biology at Akdeniz University, MS in Molecular Biology atIzmir Institute of Technology. Pursuing PhD at Department ofMedical Biology, Ondokuz Mayis University.


Klappentext

In this study, expression profiles of the toxic geneHSV-1 TK on parental HeLa cells and HeLa cellsexpressing HIV-1 regulatory genes was desired to beassessed. The toxic gene (suicide gene) presumed tocreate the desired effect was placed under thetranscriptional control of HIV promoter LTR and sothat the expression of the toxic gene was madedependent upon the tat regulator gene of HIV. Inorder to prevent leaky gene expression stemming fromthe basal gene expression from LTR even if it was notinduced by Tat, and thereby having potential todamage healthy cells, the prerequisite cis-acting DNAsequences were cloned downstream of the toxic gene.So that, the transcripts produced could retain in thenucleus and would require the function of a secondregulator protein 'Rev' for being transmitted intothe cytoplasm. However, since the generation ofstable cell lines expressing Tat and Rev proteinscould not be achieved, the possibility of workingwith the suicide vector constructed for this purposewas out of question. In this book, the strategies andthe handicaps will be discussed for researchers whoaim to develop cloning based approaches to preventHIV infection.

Cart 30 Tage Rückgaberecht
Cart Garantie

Weitere Informationen

  • Allgemeine Informationen
    • GTIN 09783639151947
    • Sprache Deutsch
    • Größe H220mm x B220mm
    • Jahr 2009
    • EAN 9783639151947
    • Format Kartonierter Einband (Kt)
    • ISBN 978-3-639-15194-7
    • Titel A Suicide Vector Design for Cells Expressing HIV-1 Regulatory Genes
    • Autor Zeynep Ye in
    • Untertitel Basics, Methods, Handicaps
    • Herausgeber VDM Verlag
    • Anzahl Seiten 136
    • Genre Biologie

Bewertungen

Schreiben Sie eine Bewertung
Nur registrierte Benutzer können Bewertungen schreiben. Bitte loggen Sie sich ein oder erstellen Sie ein Konto.