Cellulases: Volume 510

Klappentext Cellulase refers to a class of enzymes produced chiefly by fungi, bacteria, and protozoans that catalyze cellulolysis. This volume of Methods in Enzymology comprehensively covers this topic. With an international board of authors, this volume covers subjects such as "The DNSA reducing assay for measuring cellulases," "Measuring processivity" and "In situ cellulose detection with carbohydrate-binding modules." Zusammenfassung With an international board of authors! this book covers subjects such as "The DNSA reducing assay for measuring cellulases"! "Measuring processivity" and "In situ cellulose detection with carbohydrate-binding modules". Inhaltsverzeichnis Measurement of endo-1,4-b-glucanase Biomass Conversion determined via Fluorescent Cellulose Decay assay The analysis of saccharification in biomass using an automated high-throughput method Studies of enzymatic cleavage of cellulose using Polysaccharide Analysis by Carbohydrate gel Electrophoresis (PACE) Measuring processivity Distinguishing xyloglucanase activity in endo-b (1® 4) glucanases Methods for structural characterization of the products of cellulose- and xyloglucan-hydrolyzing enzymes The crystallization and structural analysis of cellulases (and other glycoside hydrolases): strategies and tactics Visualization of Cellobiohydrolase I from Trichoderma reesei Moving on Crystalline Cellulose Using High-Speed Atomic Force Microscopy Small angle X-ray scattering and crystallography; a winning combination for exploring the multi-modular organisation of cellulolytic macromolecular complexes Quantitative approaches to the analysis of carbohydrate-binding module function In Situ Detection Of Cellulose With Carbohydrate-Binding Modules Interactions between family 3 carbohydrate binding modules (CBMs) and cellulosomal linker peptides Approaches for improving thermostability characteristics in cellulases Thermophilic glycosynthases for oligosaccharides synthesis Engineering cellulase activity into Clostridium acetobutylicum Transformation of Clostridium thermocellum by electroporation Genetic and Functional Genomic Approaches for the Study of Plant Cell Wall Degradation in Cellvibrio japonicus Methods for the isolation of cellulose-degrading microorganisms Metagenomic approaches to the discovery of cellulases Escherichia coli expression, purification, crystallization and structure determination of bacterial cohesin-dockerin complexes Measurements of relative binding of cohesin and dockerin mutants using an advanced ELISA technique for high-affinity interactions Designer cellulosomes for enhanced hydrolysis of cellulosic substrates High-throughput screening of cohesin mutant libraries on cellulose microarrays...

Zusammenfassung
With an international board of authors, this book covers subjects such as "The DNSA reducing assay for measuring cellulases", "Measuring processivity" and "In situ cellulose detection with carbohydrate-binding modules".

Inhalt

1. Measurement of endo-1,4-b-glucanase
2. Biomass Conversion determined via Fluorescent Cellulose Decay assay
3. The analysis of saccharification in biomass using an automated high-throughput method
4. Studies of enzymatic cleavage of cellulose using Polysaccharide Analysis by Carbohydrate gel Electrophoresis (PACE)
5. Measuring processivity
6. Distinguishing xyloglucanase activity in endo-b (1® 4) glucanases
7. Methods for structural characterization of the products of cellulose- and xyloglucan-hydrolyzing enzymes
8. The crystallization and structural analysis of cellulases (and other glycoside hydrolases): strategies and tactics
9. Visualization of Cellobiohydrolase I from Trichoderma reesei Moving on Crystalline Cellulose Using High-Speed Atomic Force Microscopy
10. Small angle X-ray scattering and crystallography; a winning combination for exploring the multi-modular organisation of cellulolytic macromolecular complexes
11. Quantitative approaches to the analysis of carbohydrate-binding module function
12. In Situ Detection Of Cellulose With Carbohydrate-Binding Modules
13. Interactions between family 3 carbohydrate binding modules (CBMs) and cellulosomal linker peptides
14. Approaches for improving thermostability characteristics in cellulases
15. Thermophilic glycosynthases for oligosaccharides synthesis
16. Engineering cellulase activity into Clostridium acetobutylicum
17. Transformation of Clostridium thermocellum by electroporation
18. Genetic and Functional Genomic Approaches for the Study of Plant Cell Wall Degradation in Cellvibrio japonicus
19. Methods for the isolation of cellulose-degrading microorganisms
20. Metagenomic approaches to the discovery of cellulases
21. Escherichia coli expression, purification, crystallization and structure determination of bacterial cohesin-dockerin complexes
22. Measurements of relative binding of cohesin and dockerin mutants using an advanced ELISA technique for high-affinity interactions
23. Designer cellulosomes for enhanced hydrolysis of cellulosic substrates
24. High-throughput screening of cohesin mutant libraries on cellulose microarrays