Chemical and Synthetic Biology Approaches to Understand Cellular Functions - Part A

Chemical and Synthetic Biology Approaches to Understand Cellular Functions - Part A, Volume 621, the latest release in the Methods in Enzymology series, highlights new advances in the field, with this volume covering Site-directed ethylation of membrane proteins for measuring conformational transitions in lipid bilayers, the Design and synthesis of fluorescent activity probes for protein phosphatases, Stains, Utilizing split-nanoLuc fragments as luminescent probes for protein solubility in living cells, SH2-domain based sensor for intracellular recognition of sulfo-tyrosine, DNA-encoded immunoglobulins for detection of parasites, An engineered TEV protease - calmodulin fusion based sensor for neuronal calcium recording, and much more.

Autorentext
Dr. Arun K. Shukla is a world leader in the field of GPCR biology and he is currently a Professor in the Department of Biological Sciences and Bioengineering at the Institute of Technology, Kanpur in India. Dr. Shukla's research program is focused on understanding the structure, function and regulation of G protein-coupled receptors with a long-term of designing novel therapeutics with minimized side-effects.

Inhalt

1. Targeted m6A reader proteins to study the epitranscriptome
   Simone Rauch and Bryan C. Dickinson
   2. Synthetic gene expression circuits regulating sexual reproduction
   Nobuo Fukuda and Shinya Honda
   3. Nucleotide resolution sequencing of N4-acetylcytidine in RNA
   Justin M. Thomas, Keri M. Bryson and Jordan L. Meier
   4. A guide for drug inducible genome editing with HIT systems
   Chen Zhao, Shixian Wei and Yu Wang
   5. A guide for drug inducible transcriptional activation with HIT systems
   Chen Zhao, Shixian Wei and Yu Wang
   6. Direct cloning and heterologous expression of natural product biosynthetic gene clusters by transformation-associated recombination
   Jia Jia Zhang, Kazuya Yamanaka, Xiaoyu Tang and Bradley S. Moore
   7. Salt-sensitive intein for large-scale polypeptide production
   Yi-Zong Lee and Shih-Che Sue
   8. Methods for the recombinant expression of active tyrosine kinase domains: Guidelines and pitfalls
   M. Escarlet Díaz Galicia, Abdullah Aldehaiman, SeungBeom Hong, Stefan T. Arold and Raik Grünberg
   9. Design, cloning and characterization of transcription factor-based inducible gene expression systems
   Erik K.R. Hanko, Nigel P. Minton and Naglis Malys
   10. Design, construction, and validation of optogenetic proteins
   Colin P. O'Banion, Anwesha Goswami and David S. Lawrence
   11. Overcoming component limitations in synthetic biology through transposon-mediated protein engineering
   Joshua T. Atkinson, Bingyan Wu, Laura Segatori and Jonathan J. Silberg
   12. Chemical biology of glycoproteins: From chemical synthesis to biological impact
   Yaohao Li, Amy H. Tran, Samuel J. Danishefsky and Zhongping Tan
   13. In bulla functional channel expression systems that mimic bacterial synthetic membranes
   Masayuki Iwamoto and Shigetoshi Oiki
   14. A mass spectrometry-based isotope-coded mass tag method to map thiol accessibility in biological systems
   John E. Gadbery and Nicole S. Sampson
   15. Quick-soaking of crystals reveals unprecedented insights into the catalytic mechanism of glycosyltransferases
   David Albesa-Jové, Javier O. Cifuente, Beatriz Trastoy and Marcelo E. Guerin
   16. Cysteine-ethylation of tissue-extracted membrane proteins as a tool to detect conformational states by solid-state NMR spectroscopy
   Daniel K. Weber, Taysir Bader, Erik K. Larsen, Songlin Wang, Tata Gopinath, Mark Distefano and Gianluigi Veglia
   17. Improved sensitivity and resolution of in-cell NMR spectra
   David S. Burz, Leonard Breindel and Alexander Shekhtman
   18. High-throughput methods in aptamer discovery and analysis
   Kyle H. Cole and Andrej Lupták
   19. Drop-in-well chamber for droplet interface bilayer with built-in electrodes
   Kazuhiro Urakubo, Masayuki Iwamoto and Shigetoshi Oiki