Chemical Microbiology Part B: Volume 665

Chemical Microbiology, Part B, Volume 665, the latest release in the Methods of Enzymology series, highlights new advances in the field, including comprehensive chapters on the Application of Antibiotic-derived Fluorescent Probes to Bacterial Studies, Metabolomic approaches for enzyme function and pathway discovery in bacteria, Adding a diazo-transfer reagent to culture to generate secondary metabolite probes for click chemistry, Customized Peptidoglycan Surfaces to Investigate Innate Immune Recognition via Surface Plasmon Resonance, Development and application of highly sensitive labeling reagents for amino acids, Bacterial Cell Wall Modification with a Glycolipid Substrate, and much more.

Autorentext
Erin E. Carlson received her B.A. at St. Olaf College (Northfield, MN) in 2000. She went on to graduate studies funded by the NIH Predoctoral Biotechnology Training Program at the University of Wisconsin - Madison and earned a Ph.D. in organic chemistry in 2005 under the direction of Professor Laura L. Kiessling. Her graduate career focused on the design and synthesis of mechanistic probes and inhibitors for carbohydrate-binding proteins, concentrating on the study of UDP-galactopyranose mutase (UGM), an enzyme involved in cell wall biosynthesis of Mycobacterium tuberculosis.

Subsequently, Dr. Carlson was awarded an American Cancer Society Postdoctoral Fellowship for studies at The Scripps Research Institute with Professor Benjamin F. Cravatt. Carlson and Cravatt developed a global metabolite profiling strategy that utilizes chemoselective probes to enable enrichment and profiling of metabolites from complex biological systems. This technology, referred to as Metabolite Enrichment by Tagging and Proteolytic Release (METPR), facilitates the rigorous characterization of biochemical pathways through their most sensitive reporter, endogenous small molecules. In 2007, Dr. Carlson received an NIH Pathway to Independence Award (K99/R00) and joined the faculty at Indiana University in 2008. In the summer of 2014, she joined the faculty in the Chemistry Department at the University of Minnesota and was appointed as a Graduate Faculty member of the Department of Medicinal Chemistry, the Department of Biochemistry, Molecular Biology and Biophysics and the graduate program in Biomedical Informatics and Computational Biology in 2015.

Since the start of her independent career, she has won numerous awards including being named a Presidential Early Career Awards for Scientists and Engineers (PECASE) recipient, a Pew Biomedical Scholar, the NIH Director's New Innovator Award, the Indiana University Outstanding Junior Faculty Award, the NSF CAREER Award, the Cottrell Scholar Award and was named a Sloan Research Fellow and an Indiana University Dean's Fellow. Professor Carlson has been highlighted in several videos including one produced by NBC in their Science Behind The News series, a fast-paced video series supported by the National Science Foundation. This piece was one of five videos highlighting work funded by NSF's Directorate for Mathematical and Physical Sciences. She was also featured in a "Brilliant Minds" video. Dr. Carlson was also named one of "Tomorrow's PIs" in the sixth annual issue of Genome Technology and received the Outstanding Postdoctoral Mentor Award from the University of Minnesota Postdoctoral Association in 2017.

Inhalt

1. Application of Antibiotic-derived Fluorescent Probes to Bacterial Studies
2. Metabolomic approaches for enzyme function and pathway discovery in bacteria
3. Adding a diazo-transfer reagent to culture to generate secondary metabolite probes for click chemistry
4. Customized Peptidoglycan Surfaces to Investigate Innate Immune Recognition via Surface Plasmon Resonance
5. Development and application of highly sensitive labeling reagents for amino acids
6. Bacterial Cell Wall Modification with a Glycolipid Substrate
7. Screening an Established Natural Product Library Identifies Secondary Metabolites that Potentiate Conventional Antibiotics
8. Reactivity-Based Screening for Natural Product Discovery
9. Applications of an Inactive Dispersin B Probe to Monitor Biofilm Polysaccharide Production
10. Synthesis and Application of Fluorescent Teixobactin Analogues
11. Impact of Crossbridge Structure on Peptidoglycan Crosslinking: A Synthetic Stem Peptide Approach
12. Utilizing Imaging Mass Spectrometry to Analyze Microbial Biofilm Chemical Responses to Exogenous Compounds
13. Guidelines for Metabolomics-Guided Transposon Mutagenesis for Microbial Natural Product Discovery
14. Glycopeptide antibiotic discovery in the genomic era
15. Engineered Biosynthesis of Alkyne-tagged Polyketides**