Contemporary Approaches to the Study of Pain

This volume contains experimental approaches that are currently revolutionizing our understanding of the neurobiology of pain. The chapters cover many cutting-edge methods including the identification of gene expression profiles, transcriptomes or translatomes, from individual cells or defined groups of cells in rodents and primates; the electrophysiological investigation of human tissues, such as human dorsal root ganglion neurons; ways to assess modality response profiles of neurons using calcium imaging in vitro and in vivo; and somatosensory behaviors in rodents using high-speed videography and machine learning. In the Neuromethods series style, the chapters include detailed advice from specialists to obtain successful results in your laboratory.

Cutting-edge and comprehensive, Contemporary Approaches to the Study of Pain: From Molecules to Neural Networks is a valuable resource for scientists and researchers interested in making impactful contributions to our understanding of pain.

Includes cutting-edge methods and protocols Provides step-by-step detail essential for reproducible results Contains key notes and implementation advice from the experts

Inhalt
Single-Cell RNA-Sequencing of Somatosensory Neurons.- Preparation of Human and Rodent Spinal Cord Nuclei for Single-Nucleus Transcriptomic Analysis.- Multiplex In Situ Hybridization of the Primate and Rodent DRG and Spinal Cord.- Using Translating Ribosome Affinity Purification (TRAP) to Understand Cell-Specific Translatomes in Pain States.- Ex Vivo Skin-Teased Fiber Recordings from Tibial Nerve.- Single Unit Electrophysiological Recordings of Primary Muscle Sensory Neurons using a Novel Ex Vivo Preparation.- Electrophysiological Recording Techniques from Human Dorsal Root Ganglion.- Human Pluripotent Stem Cell-Derived Sensory Neurons: A New Translational Approach to Study Mechanisms of Sensitization?.- Intraspinal Transplantation of Precursors of Cortical GABAergic Interneurons to Treat Neuropathic Pain.- A Co-Culture System for Studying Dorsal Spinal Cord Synaptogenesis.- Visualizing Synaptic Connectivity using Confocal and Electron Microscopy: Neuroanatomical Approaches to Define Spinal Circuits.- Using Viral Vectors to Visualize Pain-Related Neural Circuits in Mice.- Recording Pain-Related Brain Activity in Behaving Animals using Calcium Imaging and Miniature Microscopes.- Optical Imaging of the Spinal Cord for the Study of Pain: From Molecules to Neural Networks.- In Vivo Calcium Imaging of Peripheral Ganglia.- Optogenetic Modulation of the Visceromotor Response to Reveal Visceral Pain Mechanisms.- Use of Optogenetics for the Study of Skin-Nerve Communication.- Channelrhodopsin-2 Assisted Circuit Mapping in the Spinal Cord Dorsal Horn.- Production of AAVs and Injection into the Spinal Cord.- The Use of Intraspinally Injected Chemogenetic Receptor, PSAM-GlyR, to Probe the Functional Role of Spinal Dorsal Horn Neurons.- Measuring Mouse Somatosensory Reflexive Behaviors with High-Speed Videography, Statistical Modeling, and Machine Learning.