Developmental Toxicology

This book details methods used to study developmental toxicology. It highlights the evolution of methods from classical teratology approaches to dynamic, state-of-the-art molecular methods, systems biology, and next generation models and procedures.

The discipline of developmental toxicology is an integration of concepts, models, and methodologies based heavily on the superimposition of toxicology principles upon the science of developmental biology. The science of developmental toxicology also borrows from other research areas that are concerned with regulation of cell growth, migration, differentiation and cell death, as such are central to the study of stem cells, cancer, and chronic diseases. In Developmental Toxicology: Methods and Protocols expert researchers in the field detail many of the methods which are now commonly used to study developmental toxicology highlighting the evolution of methods from classical teratology approaches to the dynamic, state-of-the-art molecular methods, systems biology, and next generation models and procedures. Written in the highly successful Methods in Molecular Biology™ series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and key tips on troubleshooting and avoiding known pitfalls.

Authoritative and practical, Developmental Toxicology: Methods and Protocols is a valuable resource to those planning experiments to investigate consequences of environmental, nutritional, or chemical effects caused during development.

Highlights the evolution of methods from classical teratology approaches to the dynamic, state-of-the-art molecular methods Provides step-by-step detail essential for reproducible results Contains key notes and implementation advice from the experts Includes supplementary material: sn.pub/extras

Inhalt

Volume Overview - Introduction.-In Vivo Models of Developmental Toxicology.-Caenorhabditis Elegans as a Model in Developmental Toxicology.-Zebrafish Embryo Developmental Toxicology Assay.-Gene Knockdown by Morpholino-modified Oligonucleotides in the Zebrafish (Danio rerio) Model: Applications for Developmental Toxicology.-An Amphibian Model for Studies of Developmental Reproductive Toxicity.-Avian Models in Teratology and Developmental Toxicology.-Overview of In Vitro Models in Developmental Toxicology.-Primary Cell and Micromass Culture in Assessing Developmental Toxicity.-Embryonic Stem Cell Test (EST): Stem cell Use in Predicting Developmental Cardiotoxicity and Osteotoxicity.-Mouse Embryonic Stem Cell Adherent Cell Differentiation and Cytotoxicity (ACDC) Assay.-The Murine Limb Bud in Culture as an In Vitro Teratogenicity Test System.-Rodent Whole Embryo Culture.-Rabbit Whole Embryo Culture.-Assessment of Xenobiotic Biotransformation Including Reactive Oxygen Species Generation in the Embryo using Benzene as an Example.-Methodological Approaches to Cytochrome P450 Profiling in Embryos.-Analysis of Nrf2-Mediated Transcriptional Induction of Antioxidant Response in Early Embryos.-Regulation and Control of AP-1 Binding Activity in Embryotoxicity.-Thioredoxin Redox Status Assessment During Embryonic Development: The Redox Western.-Methods for the Determination of Plasma or Tissue Glutathione Levels.-Oxidative Stress, Thiols, and Redox Profiles.-A Review of Toxicogenomic Approaches in Developmental Toxicology.-Epigenetic Approaches and Methods in Developmental Toxicology: Role of HDAC Inhibition in Teratogenic Events.-DNA Methylation Screening and Analysis.-Assessment of Histiotrophic Nutrition using Fluorescent Probes.-Diabetic Embryopathy.-Gene Expression Analysis in Developing Embryos: In situ Hybridization.-Assessment of Gross Fetal Malformations: The Modernized Wilson Technique and Skeletal Staining.