Digital Holography Microscopy applications

This book describes general and robust algorithms
that are devoted to automate the analysis process in
the 3D space and in time of dynamic objects present
in a volume studied by a specific microscope that
permits to record images containing their 3D
information. This imaging system using a coherent
light records not only the intensity information of
the light, but also the direction of propagation for
each pixel in the image. Together with a numerical
model for coherent light diffraction, it is possible
to generate numerically other images parallel to the
recorded plane and as a consequence to refocus
numerically objects. The acquisition time of 3D data
is as a consequence only limited by the acquisition
rate of the CCD camera of in the imaging system.
The extraction of 3D information is performed by
algorithm applied on the recorded data. The studied
objects are either of biological nature or latex
particles. A focus criterion to determine the best
focus plane of an object is proposed. From the
knowledge of the 3D position of objects, several
analyses are proposed like shape or object
trajectories analysis in time and in 3D.

Autorentext

1997 - 2002: electrical civil engineer Université Catholique de Louvain with honors. 2004 - 2005: specialized study in image processing Université Libre de Bruxelles (ULB) with the highest honors. 2004 - 2007: (ULB) PhD thesis in image processing (digital holography) and optics for the European Space Agency.

Klappentext

This book describes general and robust algorithms that are devoted to automate the analysis process in the 3D space and in time of dynamic objects present in a volume studied by a specific microscope that permits to record images containing their 3D information. This imaging system using a coherent light records not only the intensity information of the light, but also the direction of propagation for each pixel in the image. Together with a numerical model for coherent light diffraction, it is possible to generate numerically other images parallel to the recorded plane and as a consequence to refocus numerically objects. The acquisition time of 3D data is as a consequence only limited by the acquisition rate of the CCD camera of in the imaging system. The extraction of 3D information is performed by algorithm applied on the recorded data. The studied objects are either of biological nature or latex particles. A focus criterion to determine the best focus plane of an object is proposed. From the knowledge of the 3D position of objects, several analyses are proposed like shape or object trajectories analysis in time and in 3D.