Fluorescence In-Situ Hybridization (Fish) for Microbial Cells

This volume provides a comprehensive review of concepts and protocols related to fluorescence in situ hybridization (FISH) applied to microbial cells. Chapters will serve guide for the design of new probes and the development of novel FISH-based protocols. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.

Authoritative and cutting-edge, Fluorescence In Situ Hybridization (FISH) for Microbial Cells: Methods and Concepts **aims to ensure successful results in the further study of this vital field.

Includes cutting-edge methods and protocols Provides step-by-step detail essential for reproducible results Contains key notes and implementation advice from the experts

Inhalt
An Introduction to Fluorescence in situ Hybridization in Microorganisms.- FISH Variants.- Bioinformatic Tools and Guidelines for the Design of Fluorescence in situ Hybridization Probes.- FISH in Suspension or in Adherent Cells.- Application of Nucleic Acid Mimics in Fluorescence in situ Hybridization.- Delivery of Oligonucleotides into Bacteria by Fusogenic Liposomes.- Characterisation of Social Interactions and Spatial Arrangement of Individual Bacteria in Multi-Strain or Multispecies Biofilm Systems using Nucleic acid mimics-Fluorescence in situ Hybridization.- Leaf-FISH: In Situ Hybridization Method for Visualizing Bacterial Taxa on Plant Surfaces.- CAtalyzed Reporter Deposition Fluorescence In Situ Hybridization (CARD-FISH) for Complex Environmental Samples.- Fluorescence in Situ Hybridization with Quantum Dot Labels in E. coli Cells.- Monitoring Bacteriophage Infection on Bacterial Cells using FISH.- Linking Microbes to their Genes at Single Cell Level with directgeneFISH.- Assigning Function To Phylogeny: FISH-nanoSIMS.- Assigning Function to Phylogeny: MAR-FISH.- Counting mRNA Copies in Intact Bacterial Cells by Fluctuation Localization Imaging-based Fluorescence in situ hybridization (fliFISH).- Integration of FISH and Microfluidics.- Flow-FISH using Nucleic Acid Mimic Probes for the Detection of Bacteria.- FISH in Food Samples.- Extraction of Microbial Cells from Environmental Samples for FISH Approaches.- Quality Control in Diagnostic Fluorescence In Situ Hybridization (FISH) in Microbiology.- Computational Resources and Strategies to Construct Single-Molecule Models of FISH.