Forensic DNA Typing Protocols

A state-of-the-art collection of readily reproducible laboratory methods for DNA identity analysis, including Y chromosome haplotyping, mtDNA, and SNP typing. The book offers well-tested protocols for DNA quantification using real-time PCR on forensic samples and for the determination of the number of amelogenine gene copies. For forensic geneticists, there are readily reproducible methods for species identification, ancient DNA, and pharmacogenetics. Additional chapters address new applications in the forensic genetics lab, such a species identification or typing of CYP polymorphisms for the analysis of adverse to drugs.

Includes supplementary material: sn.pub/extras

Klappentext
In recent years, the rapid emergence of different markers and technologies for DNA typing has transformed the field of forensic identification with breathtaking speed. In Forensic DNA Typing Protocols, leading forensic geneticists from around the world describe in step-by-step detail the cutting-edge laboratory methods they now use for DNA identity analysis, including Y chromosome haplotyping, mtDNA, and SNP typing. The book offers well-tested protocols for DNA quantification using real-time PCR on forensic samples and for the determination of the number of amelogenine gene copies. Additional chapters address new applications in the forensic genetics lab, such a species identification, ancient DNA, or typing of CYP polymorphisms for the analysis of adverse to drugs. The protocols follow the successful Methods in Molecular Biology™ series format, each offering step-by-step laboratory instructions, an introduction outlining the principle behind the technique, lists of the necessary equipment and reagents, and tips on troubleshooting and avoiding known pitfalls.
Up-to-date and highly practical, Forensic DNA Typing Protocols captures and makes practical for all laboratories the whole array of new technologies and techniques that have revolutionized forensic science.

Inhalt
Forensic DNA-Typing Technologies.- DNA Extraction and Quantitation of Forensic Samples Using the Phenol-Chloroform Method and Real-Time PCR.- A Real-Time PCR Protocol to Determine the Number of Amelogenin (X-Y) Gene Copies From Forensic DNA Samples.- Species Determination.- Constructing STR Multiplex Assays.- Y Chromosome STR Typing.- Using Online Databases for Developing SNP Markers of Forensic Interest.- SNP Typing in Forensic Genetics.- Genotyping SNPs With the LightCycler.- Universal Tag Arrays in Forensic SNP Analysis.- SNP Typing on the NanoChip Electronic Microarray.- Genotyping SNPs Using a UV-Photocleavable Oligonucleotide in MALDI-TOF MS.- Mitochondrial D-Loop and Coding Sequence Analysis Using Pyrosequencing.- SNaPshot Typing of Mitochondrial DNA Coding Region Variants.- Typing of Y Chromosome SNPs With Multiplex PCR Methods.- Y Chromosome SNP Analysis Using the Single-Base Extension.- SNaPshot for Pharmacogenetics by Minisequencing.- Methods for the Study of Ancient DNA.- Protocols for Ancient DNA Typing.