Glycoanalysis Protocols

Now fully updated and considerably expanded, Glycoanalysis Protocols, 2nd ed., makes available to all protein scientists, and particularly those working with today's pharmaceuticals, the most advanced and reproducible glycoanalysis techniques currently in use. Developed by highly experienced carbohydrate chemists, biochemists, and physical chemists, these detailed, up-to-date, and proven analytical techniques cover the areas of glycoprotein macromolecular structural analysis, oligosaccharide profiling, lipid conjugate characterization, microorganism structure determination, and proteoglycan function. Special attention has been given to advanced analytical techniques in biotechnology during the production of recombinant glycoproteins and other therapeutics.

Hailed as "indispensable" in its first edition, Glycoanalysis Protocols, 2nd ed., continues with vital, time-tested techniques addressing the needs of both biomedical researchers and protein macromolecular structural chemists. It will well serve all those starting work on the analysis of glycoproteins, as well as more experienced investigators seeking to augment their expertise.

Klappentext

Glycoanalysis Protocols, 2nd ed., makes available to all protein scientists, and particularly those working in today's pharmaceuticals industry, the most advanced and reproducible glycoanalysis techniques. These detailed, up-to-date, and proven analytical methods cover the areas of glycoprotein macromolecular structural analysis, oligosaccharide profiling, lipid conjugate characterization, microorganism structure determination, and proteoglycan function. They also include advanced analytical techniques in biotechnology during the production of recombinant glycoproteins and other therapeutics. These protocols will well serve anyone starting work on the analysis of glycoproteins, as well as experienced investigators seeking to carry their expertise to higher levels of accomplishment.

Inhalt
Characterization of Protein Glycosylation.- Analytical Methods for the Study of O-GlcNAc Glycoproteins and Glycopeptides.- Analysis of Asparagine-Linked Oligosaccharides by Sequential Lectin-Affinity Chromatography.- Exoglycosidase Sequencing of N-Linked Glycans by the Reagent Array Analysis Method (RAAM).- HPAEC-PAD Analysis of Monosaccharides Released by Exoglycosidase Digestion Using the Carbopac MA1 Column.- HPLC and HPAEC of Oligosaccharides and Glycopeptides.- Analysis of N- and O-Glycans by Pyridylamination.- Polyacrylamide Gel Electrophoresis of Fluorophore-Labeled Carbohydrates from Glycoproteins.- Analysis of Glycosaminoglycans and Proteoglycans.- The Use of Cell and Organ Culture for the Study of Secreted Mucins.- Purification of Gastrointestinal Mucins and Analysis of Their O-Linked Oligosaccharides.- TLC in Structure and Recognition Studies of Glycosphingolipids.- Strategies for Preliminary Characterization of Novel Amphoteric Glycosphingolipids.- Analysis of the Carbohydrate and Lipid Components of Glycosylphosphatidylinos¡tol Structures.- Conformational Analysis of Biantennary Glycopeptides with a Resonance Energy Transfer Technique.