Lung Cancer

The Methods in Molecular Medicine series is intended as a resource for both novice and experienced investigators attempting to diversify their tech- logical base in research. Lung Cancer: Volume 1: Molecular Pathology Me- ods and Reviews presents an overview of the current status of assays employed to detect and characterize the multitude of pathologies that contribute to the development of this deadly disease. As with all volumes in the Methods in Molecular Medicine series, the reader should find that each methods-based chapter provides clear instructions for the performance of various protocols, supplemented by additional technical notes that provide valuable insight. These notes are designed to enable the reader to acquire the techniques described with a proficiency not easily achieved by re- ing standard method formats. No volume can exhaustively cover every aspect of biological research and there will be gaps in this endeavor that one or another research group will id- tify. Each section herein could readily be expanded into a book in its own right. However, I have sought to include a spectrum of techniques that should allow for the acquisition of key skills in each area covered.

Includes supplementary material: sn.pub/extras

Klappentext
Molecular-based laboratory methods can help significantly in the diagnosis and treatment of lung cancer, a leading cause of death worldwide. In Lung Cancer, Volume 1: Molecular Pathology Methods and Reviews, leading physicians, scientists, and noted researchers review novel methods for determining the etiology of a variety of lung cancers and present readily reproducible techniques for examining the associated multitude of genetic abnormalities. The methods described make it possible to detect these alterations at the cellular, DNA, and protein levels, to study the development of lung cancer in vitro and in vivo-either in situ or in the form of metastases-and to test targeted therapies with detailed model systems. An animal models section gives explicit instructions for setting up and testing these systems, which are rarely described in the literature. A companion volume, Lung Cancer, Volume 2: Diagnostic and Therapeutic Methods and Reviews, concentrates on diagnosing lung cancer through molecular analysis of clinical samples and providing a variety of novel therapies.
Comprehensive and informative, Lung Cancer, Volume 1: Molecular Pathology Methods and Reviews illuminates the etiology of lung cancer and provides reliable techniques for detecting the responsible genetic mutations at the cellular, DNA, and protein levels. Together, the two volumes constitute an unprecedented resource for those who wish to better understand how this disease is being diagnosed and treated and what novel techniques may be available to improve both processes.

Inhalt
Characteristic Genetic Alterations in Lung Cancer.- Etiology and Classification of Lung Tumors.- Clinical and Biological Relevance of Recently Defined Categories of Pulmonary Neoplasia.- Molecular Epidemiology of Human Cancer Risk.- Neuroendocrine Phenotype of Small Cell Lung Cancer.- AIDS-Associated Pulmonary Cancers.- Molecular Abnormalities in Lung Cancer.- Abrogation of the RB-p16Tumor Suppressor Pathway in Human Lung Cancer.- Sensitive Detection of Hypermethylated p16INK4a Alleles in Exfoliative Tissue Material.- Role of Receptor Tyrosine Kinases in Lung Cancer.- Relationship of EGFR Signal-Transduction Modulation by Tyrosine Kinase Inhibitors to Chemosensitivity and Programmed Cell Death in Lung Cancer Cell Lines.- Detection of the Transcripts and Proteins for the Transforming Growth Factor-? Isoforms and Receptors in Mouse Lung Tumorigenesis Using In Situ Hybridization and Immunohistochemistry in Paraffin-Embedded Tissue Sections.- Alterations in the Expression of Insulin-Like Growth Factors and Their Binding Proteins in Lung Cancer.- Screening of Mutations in the ras Family of Oncogenes by Polymerase Chain Reaction-Based Ligase Chain Reaction.- Assays for Raf-1 Kinase Phosphorylation and Activity in Human Small Cell Lung Cancer Cells.- ?-Glutamylcysteine Synthetase in Lung Cancer.- Localization of Cyclooxygenase-2 Protein Expression in Lung Cancer Specimens by Immunohistochemical Analysis.- Non-RI Protocols for L-myc Allelotyping and Deletion Mapping of Chromosome 1p in Primary Lung Cancers.- Intercellular Adhesion Molecules (ICAM-1, VCAM-1, and LFA-1) in Adenocarcinoma of Lung.- MUC1 Expression in Lung Cancer.- Altered Surface Markers in Lung Cancer.- Prognostic Relevance of Angiogenic, Proliferative, and Apoptotic Factors in Lung Carcinomas.- MorphologicalAssessment of Apoptosis in Human Lung Cells.- [D-Arg6, D-Trp7,9, NmePhe8]-Substance P (6-11) Activates JNK and Induces Apoptosis in Small Cell Lung Cancer Cells via an Oxidant-Dependent Mechanism.- Isolation of Cells Entering Different Programmed Cell Death Pathways Using a Discontinuous Percoll Gradient.- Angiogenesis, Metastasis, and Lung Cancer.- Matrix Metalloproteinase Expression in Lung Cancer.- Vascular Endothelial Growth Factor Expression in Non-Small Cell Lung Cancer.- Regulation of Angiostatin Mobilization by Tumor-Derived Matrix Metalloproteinase-2.- In Vitro and In Vivo Assays for the Proliferative and Vascular Permeabilization Activities of Vascular Endothelial Growth Factor (VEGF) and Its Receptor.- Detection of Telomerase Activity in Lung Cancer Tissues.- Analysis of Alterations in a Base-Excision Repair Gene in Lung Cancer.- Lung Cancer Model Systems.- Induction of Lung Cancer by Passive Smoking in an Animal Model System.- Metastatic Orthotopic Mouse Models of Lung Cancer.- Lung-Specific Expression of Mutant p53 as Mouse Model for Lung Cancer.- Use of Nucleotide Excision Repair-Deficient Mice as a Model For Chemically Induced Lung Cancer.- Nude Mouse Lung Metastases Models of Osteosarcoma and Ewing 's Sarcoma for Evaluating New Therapeutic Strategies.- Tumor-Specific Metastasis to Lung Using Reporter Gene-Tagged Tumor Cells.- Cultures of Surgical Material from Lung Cancers.- The Hollow Fiber Assay.