Lung Cancer

This work, Lung Cancer, Volume 2: Diagnostic and Therapeutic Methods and Reviews, in the Methods in Molecular Medicine series presents an overview of the current status of those methods useful in the diagnosis and tre- ment of lung cancerboth as it exists in the clinic and as it is being revoluti- ized in the laboratory. The book is intended to serve as a resource for researchers wishing to increase their knowledge of current and cutting edge technologies, in order that their investigations into neoplasms of the lung may benefit from this enriched diversity of techniques and approaches. Owing to the complex nature of the disease and the variety of methods available to analyze and attack it, no volume attempting to define diagnostic and therapeutic approaches to lung cancer can ever be complete. The sheer number of investigators involved in lung cancer research guarantees that some aspect will be inadvertently excluded. However, I hope that the range of techniques included herein will serve to open up new avenues of investigation for both the novice and experienced researcher.

Includes supplementary material: sn.pub/extras

Klappentext
Molecular-based laboratory methods can help significantly in the diagnosis and treatment of lung cancer, a leading cause of death worldwide. In Lung Cancer, Volume 2: Diagnostic and Therapeutic Methods and Reviews, leading physicians, scientists, and noted researchers describe step-by-step techniques for lung cancer diagnosis based on the molecular analysis of clinical samples. These experts review currently accepted treatments and outline a variety of novel therapies, including treatments based on gene therapy, immunotherapy, and chemoprevention, and show how these newly improved techniques can give a better appreciation of the prognosis associated with the particular mutation uncovered by the technique. These methods use a variety of molecular techniques, including the manipulation of gene expression and the alteration of immune status, to combat lung cancer. A companion volume, Lung Cancer, Volume 1: Molecular Pathology Methods and Reviews, illuminates the etiology of lung cancer and provides reliable techniques for detecting the responsible genetic mutations at the cellular, DNA, and protein levels.
Comprehensive and informative, Lung Cancer, Volume 2: Diagnostic and Therapeutic Methods and Reviews illuminates how molecular approaches can and are being applied to the diagnosis of lung cancer. Together, these two volumes constitute an unprecedented resource for those who wish to better understand how this disease is being diagnosed and treated, and what novel techniques may be available to improve both processes.

Inhalt
Molecular and Genetic Aspects of Lung Cancer.- Detection of Altered Tumor Markers in Clinical Samples for Diagnosis and Prognosis.- Molecular Alterations in Lung Cancer.- Cellular Predictive Factors of Drug Resistance in Non-Small Cell Lung Carcinomas.- Clinical Implications of p53 Mutations in Lung Cancer.- An Epidemiologic and Clinicopathologic Overview of AIDS-Associated Pulmonary Kaposi's Sarcoma.- Myeloperoxidase Promoter Region Polymorphism and Lung Cancer Risk.- Clinical Utility of Tumor Markers in the Management of Non-Small Cell Lung Cancer.- Detection of Chromosomal Aberrations in Lung Tissue and Peripheral Blood Lymphocytes Using Interphase Fluorescence In Situ Hybridization (FISH).- In Situ Analysis of Telomerase RNA Gene Expression as a Marker for Tumor Progression.- A High-Throughput Methodology for Identifying Molecular Targets Overexpressed in Lung Cancers.- Expression Profiling of Lung Cancer Based on Suppression Subtraction Hybridization (SSH).- Comparative Genomic Hybridization of Human Lung Cancer.- Sensitive Assays for Detection of Lung Cancer.- Fluorescent Microsatellite Analysis in Bronchial Lavage as a Potential Diagnostic Tool for Lung Cancer.- Southern Blotting of Genomic DNA from Lung and Its Tumors.- Assessment of Insulin-Like Growth Factors and Mutagen Sensitivity as Predictors of Lung Cancer Risk.- Comparative Multiplex PCR and Allele-Specific Expression Analysis in Human Lung Cancer.- Detection of K-ras Point Mutations in Sputum from Patients with Adenocarcinoma of the Lung by Point-EXACCT.- Detection of K-ras and p53 Mutations by Mutant-Enriched PCR-RFLP.- Detection of Small Cell Lung Cancer by RT-PCR for Neuropeptides, Neuropeptide Receptors, or a Splice Variant of the Neuron Restrictive Silencer Factor.- Utilization of ThyroidTranscription Factor-1 Immunostaining in the Diagnosis of Lung Tumors.- Molecular Biologic Substaging of Stage I NSCLC Through Immunohistochemistry Performed on Formalin-Fixed, Paraffin-Embedded Tissue.- A Sensitive Immunofluorescence Assay for Detection of p53 Protein in the Sputum.- SPR1.- Determination of Biological Parameters on Fine-Needle Aspirates from Non-Small Cell Lung Cancer.- Detection and Analysis of Lung Cancer Cells from Body Fluids Using a Rare Event Imaging System.- Reconstruction of Geno-Phenotypic Evolutionary Sequences from Intracellular Patterns of Molecular Abnormalities in Human Solid Tumors.- Novel Therapies.- Surgical Treatment of Lung Cancer.- Recent Advances and Dilemmas in the Radiotherapeutic Management of Locally Advanced Non-Small Cell Lung Cancer.- Photodynamic Therapy in Lung Cancer.- Gene Therapy for Lung Cancer.- Identifying Obstacles to Viral Gene Therapy for Lung Cancer.- Topical Gene Therapy for Pulmonary Diseases with PEI-DNA Aerosol Complexes.- Targeted Delivery of Expression Plasmids to the Lung via Macroaggregated Polyethylenimine-Albumin Conjugates.- Preparation of Retroviral Vectors for Cell-Cycle-Targeted Gene Therapy of Lung Cancer.- Aerosol Gene Therapy for Metastatic Lung Cancer Using PEI-p53 Complexes.- Clinical Development of Antisense Oligonucleotides as Anti-Cancer Therapeutics.- Antisense Oligonucleotides Targeting RI? Subunit of Protein Kinase A.- Use of Antisense Oligonucleotides for Therapy.- Induction of Programmed Cell Death with an Antisense Bcl-2 Oligonucleotide.- Tumor Vaccination with Cytokine-Encapsulated Microspheres.- Adoptive Immunotherapy.- Intratumoral Therapy with Cytokine Gene-Modified Dendritic Cells in Murine Lung Cancer Models.- Cyclooxygenase 2-Dependent Regulation of Antitumor Immunity in LungCancer.- Chemoprevention of Lung Cancer.- Assessing the Interaction of Particulate Delivery Systems with Lung Surfactant.- Chemopreventive Therapeutics.