Manual of Biological Markers of Disease

Section A: Methods of Autoantibody Detection
This Section provides well structured protocols for autoantibody detection. Step-by-step procedures are accompanied by explanatory notes and comments, clear diagrams, line illustrations and excellent photo illustrations. Extensive literature references lead the way to further background information. The methods presented were validated by more than 40 leading laboratories active in sera analysis, which indicates that these methods have been found to be practically useful and lead to consistent inter-laboratory results: consensus in autoantibody detection.
Section B: Autoantigens
This Section contains the first compilation of full, detailed information on autoantigens related to important autoimmune diseases. The chapters are all structured according to an easy-reference fixed template structure: specific detection methods, cellular localization, biochemical characteristics, function, cDNA and derived amino acid sequence, gene structure, B- and T-cell epitopes and lists of published monoclonal antibodies. The text is greatly enhanced by many beautiful schematic figures and photo illustrations. As in Section A, extensive literature references are provided. The information for this section was brought together by leading experts in their fields.
This Manual is an ideal text and reference book for bench scientists working in the field of autoimmunity, but also for rheumatologists, general (internal medicine) physicians or clinical immunologists caring for patients with autoimmune disorders.

Klappentext

Section A: Methods of Autoantibody Detection
This Section provides well structured protocols for autoantibody detection. Step-by-step procedures are accompanied by explanatory notes and comments, clear diagrams, line illustrations and excellent photo illustrations. Extensive literature references lead the way to further background information. The methods presented were validated by more than 40 leading laboratories active in sera analysis, which indicates that these methods have been found to be practically useful and lead to consistent inter-laboratory results: consensus in autoantibody detection.
Section B: Autoantigens
This Section contains the first compilation of full, detailed information on autoantigens related to important autoimmune diseases. The chapters are all structured according to an easy-reference fixed template structure: specific detection methods, cellular localization, biochemical characteristics, function, cDNA and derived amino acid sequence, gene structure, B- and T-cell epitopes and lists of published monoclonal antibodies. The text is greatly enhanced by many beautiful schematic figures and photo illustrations. As in Section A, extensive literature references are provided. The information for this section was brought together by leading experts in their fields.
This Manual is an ideal text and reference book for bench scientists working in the field of autoimmunity, but also for rheumatologists, general (internal medicine) physicians or clinical immunologists caring for patients with autoimmune disorders.

Inhalt

Section A: Methods of Autoantibody Detection Introduction; W.J. van Venrooij, R.N. Maini. 1. International cooperative activities in standardization of antinuclear antibodies; E.M. Tan. 2. Detection of antinuclear antibodies by immunofluorescence; R.L. Humbel. 3. Counterimmunoelectrophoresis and immunodiffusion for the detection of antibodies to soluble cellular antigens; C. Bunn, T. Kveder. 4. Protein blotting; R. Verheijen, et al. 5. Enzyme-linked immunosorbant assay in the rheumatological laboratory; P.J. Charles, R.N. Maini. 6. Immunoprecipitation of labelled proteins; E.M. Tan, C.L. Peebles. 7. Analysis of autoimmune sera by immunoprecipitation of cellular RNPs; G. Zieve, et al. 8. Measurement of antibodies to DNA; R. Smeenk. 9. Methods to detect autoantibodies to neutrophilic granulocytes; A. Wiik, et al. 10. Detection of antiperinuclear factor and antikeratin antibodies; R.M.A. Hoet. 11. Standards and reference preparations; T.E.W. Feltkamp. Section B: Autoantigens Introduction; W.J. van Venrooij, R.N. Maini. 1. Autoantigens in rheumatoid arthritis (RA). 1. RF (rheumatoid factor); R.A. Mageed. 2. APF (antiperinuclear factor); J.-M. Berthelot, et al. 3. RA-33 (hnRNP-A2); G. Steiner. 4. Collagen II; D.G. Williams. 5. The autoantigens defined by antikeratin antibodies (AKA); C. Vincent, et al. 2. Autoantigens in systemic Lupus erythematosus (SLE). 1. DNA; R.J.T. Smeenk. 2. Histones; R.W. Burlingame, R.L. Rubin. 3. Ubiquitin; S. Muller. 4. Sm; S.O. Hoch. 5. Ribosomal RNP; K.B. Elkon. 6. Ku; W.H. Reeves, et al. 7. PCNA; Y. Muro, E.M. Tan. 8. Phospholipids; E.N. Harris. 3. Autoantigens in SLE-overlap syndromes. 1. The U1 snRNP complex; J.M.T. Klein Gunnewiek, W.J. van Venrooij. 4. Autoantigens in Sjogren's syndrome. 1. Ro/SS-A; E.K.L. Chan, J.P. Buyon. 2. La/SS-B; G.J.M. Pruijn. 5. Autoantigens in Scleroderma. 1. DNA topoisomerase 1; R. Verheijen. 2. Centromere proteins; P. Verheijen. 3. Fibrillarin; R. Verheijen. 4. PM/Scl; F.A. Bautz, M. Bluhtner. 6. Autoantigens in Myositis. 1. Transfer RNA synthetases; P. Plotz, I.N. Targoff. 7. Autoantigens in Vasculitis. 1. Proteinase 3 (c-ANCA); J. Wieslander, A. Wiik. 2. Myeloperoxidase (MPO); J. Wieslander, A. Wiik. 8. Autoantigens in other diseases. 1. Mitochondrial autoantigens; P.S.C. Leung, et al. 2. P80 coilin; M. Carmo-Fonseca, et al.