Method Development and Validation for Voriconazole Estimation

A rapid, sensitive, and specific RP-HPLC method with UV detection was developed and validated for the determination of voriconazole. Chromatography was performed on an Enable C18 G column (250 × 4.6 mm) in isocratic mode, using a mobile phase of methanol and acetonitrile (60:40, v/v). The flow rate was set at 1 mL/min, and the effluent was monitored at 223 nm. Chromatographic analysis revealed a main peak for voriconazole at a retention time of 2.841 minutes. Methanol was selected as the solvent for the analysis, with the max determined to be 223 nm. The method exhibited linearity over a concentration range of 5-30 µg/mL. Validation parameters, including linearity, range, precision, accuracy, limit of detection (LOD), and limit of quantitation (LOQ), were assessed and met the required standards.

Autorentext

L'autore ha conseguito la laurea in Farmacia, il master in Farmacia e il dottorato di ricerca presso l'Università di Andhra. La sua ricerca post-dottorato presso l'Università Farmaceutica di Kyoto, in Giappone, si è concentrata sulla progettazione e sulla valutazione di sistemi di somministrazione di farmaci tramite microneedle per il rilascio controllato. Ha ricevuto il "Young Scientist Award-2016" dal CEGR di Nuova Delhi e ha vinto quattro premi per il miglior documento di ricerca.