Molecular Diagnostics for Dermatology

This user-friendly source of up-to-date information on a rapidly expanding field emphasizes the practical applications of molecular testing in the diagnosis and management of dermatological conditions. It features detailed guidance on test interpretation.

Molecular diagnostics is an exploding field, and recent advances in our understanding of the molecular basis of disease have provided a platform for the development of new diagnostic tests as well as tests to predict tumor behavior and potential response to targeted therapy. This textbook provides a reference and practical guide to molecular diagnostics for dermatologists and dermatopathologists. It outlines our current understanding of the molecular underpinnings of dermatologic disease, describes the appropriate use of currently available molecular tests, and explains the interpretation of these tests in the context of diagnosis and management. Tests relating to various disorders are covered, including but not confined to melanoma, genodermatoses, and infectious disease. Pitfalls are highlighted and user-friendly algorithmic approaches, presented.

Emphasis on practical applications of molecular testing in diagnosis and management Offers insights into the interpretation of tests Explains pitfalls and presents user-friendly algorithmic approaches Includes bullet points highlighting key information for easy application

Autorentext
Gregory A. Hosler studied chemical and biomedical engineering at Princeton University, and graduated magna cum laude in 1993. He subsequently earned his MD and PhD from the University of Texas Southwestern Medical Center before joining the Department of Pathology at The Johns Hopkins University School of Medicine in 2001. He returned to the University of Texas Southwestern Medical Center as Clinical Assistant Professor of Pathology and Dermatology in 2006. Since then, he has also worked at ProPath as a dermatopathologist and in molecular diagnostics. Dr. Hosler is an active member of the American Society of Dermatopathology, the Texas Society of Pathologists, and the American Academy of Dermatology, among others. He serves on numerous committees and regularly runs educational sessions at the annual meetings. In addition to the above speaking engagements, he has lectured at the World Congress of Dermatology and is a regular guest faculty lecturer at the annual Scripps Clinic Malignancy Update on Melanoma conference. He is on the Board of Directors of the Texas Society of Pathology and the Governing Board of ProPath. He is an editorial board member for ISRN Pathology and an ad hoc reviewer for a number of leading journals, including the Journal of Cutaneous Pathology. Dr. Hosler is the author of numerous publications in peer-reviewed journals. He is the Dermatopathology Fellowship Director at ProPath.Kathleen M. Murphy earned a PhD from the Department of Microbiology and Immunology at the University of Louisville in 1999 and subsequently completed postdoctoral training in Molecular Pathology at The Johns Hopkins University School of Medicine. She joined the faculty at The Johns Hopkins University School of Medicine in 2001, where she advanced to the rank of Associate Professor in the Departments of Pathology and Oncology. She is currently Director of Clinical Laboratory Operations at ProPath Services, Dallas, Texas and also Adjunct Associate Professor in the Department of Pathology at The Johns Hopkins University School of Medicine. Dr. Murphy has significant experience in assay design and implementation for a wide variety of molecular diagnostic applications. She has received board certification in Molecular Diagnostics from the American Board of Clinical Chemistry and has received several national awards including the American Association for Cancer Research (AACR) Young Investigator Award (1999) and the Association of Molecular Pathology (AMP) Young Investigator Award (2001). Dr. Murphy has published over 70 articles in peer-reviewed journals and is an ad hoc reviewer for a number of leading journals.

Klappentext

This textbook presents the current (and near-future) state of affairs of molecular testing as it pertains to the dermatology patient. It focuses on practical applications of molecular diagnostics over a cross-section of dermatologic disease, including melanoma, lymphoma, soft tissue tumors, genodermatoses, and infectious disease. It includes practical advice to those ordering molecular tests as well as to those considering performing such tests, providing a potential template for a comprehensive dermatologic molecular diagnostics test menu. Pitfalls of interpretation and algorithmic approaches to testing are included. The textbook is directed towards all readers clinicians, pathologists, laboratorians, and other inquisitive minds independent of their level of molecular expertise, to provoke thought or perhaps even change practice.

The context for the book is the rapid evolution of the field of molecular diagnostics, which is becoming more pervasive in all disciplines of medicine, including dermatology. This is indeed an exciting time in dermatology. Molecular testing is now incorporated into all aspects of patient management, including diagnostics (identifying and classifying disease), prognostics (predicting disease course), and theranostics (predicting response to therapy). For example, molecular tests are now used to detect germline mutations that result in genodermatoses, somatic genetic events that characterize tumors such as melanoma and sarcomas, and genetic material of otherwise undetectable infectious organisms. For melanoma and lymphoma, testing can potentially predict tumor behavior and modify patient staging. Regarding theranostics, molecular tests that identify specific mutations in proto-oncogenes, such as BRAF and others, are now used to predict which patients will respond to designer targeted therapies. Molecular theranostics has revolutionized the entiretreatment paradigm for patients with advanced melanoma, replacing excise and pray approaches with personalized medicine.

Zusammenfassung

From the book reviews: "This is an outstanding reference that thoroughly explains dysfunctional molecular pathways causing the development of skin cancer. ... The audience is dermatologists and any medical care professionals involved in treating patients with skin cancers. ... This book is a gem. Every page should be studied." (Patricia Wong, Doody's Book Reviews, January, 2015)

Inhalt
Introduction.- Terms and Definitions.- Basics of Molecular Biology and Nucleic Acids.- Principles of the Molecular Basis for Disease.- Molecular Testing Methods.- PCR and PCR-based Assays.- In situ Hybridization.- FISH and Cytogenetics.- Comparative Genomic Hybridization.- Microarray Analysis .- Melanoma.- Cutaneous Lymphoma and Leukemia Cutis.- Soft Tissue tumors.- Genodermatoses.- Infectious Disease.- Viral.- Bacterial and Mycobacterial.- Fungi and Parasites.- Emerging Molecular Applications in Dermatology.- Pharmacogenetics/Pharmacogenomics.- Theranostics.