Retinoids

Exciting new biological functions are still being discovered for vitamin A derivatives, including the myriad physiological activities of retinoids. Here, experts in the field present the latest range of techniques for both in-vitro and in-vivo studies.

Comprehensive technical guides to roles of vitamin A and retinoids in diverse physiological and pathological states and molecular pathways Up-to-date descriptions of biochemical, biophysical, and cell biological techniques Easily accessible reference volume for beginners and experts Includes supplementary material: sn.pub/extras

Inhalt
Quantification of Endogenous Retinoids.- Culture of Highly Differentiated Human Retinal Pigment Epithelium for Analysis of the Polarized Uptake, Processing, and Secretion of Retinoids.- Feeder-Independent Culture of Mouse Embryonic Stem Cells Using Vitamin A/Retinol.- In Vitro Assays of Rod and Cone Opsin Activity: Retinoid Analogs as Agonists and Inverse Agonists.- Physiological Studies of the Interaction Between Opsin and Chromophore in Rod and Cone Visual Pigments.- Measurement of the Mobility of All-Trans-Retinol with Two-Photon Fluorescence Recovery After Photobleaching.- Microfluorometric Measurement of the Formation of All-Trans-Retinol in the Outer Segments of Single Isolated Vertebrate Photoreceptors.- HPLC?/?MSN Analysis of Retinoids.- Binding of Retinoids to ABCA4, the Photoreceptor ABC Transporter Associated with Stargardt Macular Degeneration.- Fluorescence-Based Technique for Analyzing Retinoic Acid.- The Interaction Between Retinol-Binding Protein and Transthyretin Analyzed by Fluorescence Anisotropy.- Assay of Retinol-Binding ProteinTransthyretin Interaction and Techniques to Identify Competing Ligands.- Molecular Biology and Analytical Chemistry Methods Used to Probe the Retinoid Cycle.- Visualization of Retinoid Storage and Trafficking by Two-Photon Microscopy.- Reverse-Phase High-Performance Liquid Chromatography (HPLC) Analysis of Retinol and Retinyl Esters in Mouse Serum and Tissues.- Detection of Retinoic Acid Catabolism with Reporter Systems and by In Situ Hybridization for CYP26 Enzymes.- Diet in Vitamin A Research.- Experimental Approaches to the Study of A2E, a Bisretinoid Lipofuscin Chromophore of Retinal Pigment Epithelium.- Analysis of the Retinoid Isomerase Activities in the Retinal Pigment Epithelium and Retina.- Techniques to StudySpecific Cell-Surface Receptor-Mediated Cellular Vitamin A Uptake.