RNA Helicases: Volume 511

Klappentext RNA helicases have fascinated and puzzled investigators for the three decades that have passed since the first report on an RNA helicase, as a "protein involved in ATP-dependent binding of mRNA." Since then, it has become clear that RNA helicases are ubiquitous, highly conserved enzymes that participate in virtually all aspects of RNA metabolism from bacteria to human. In eukaryotic RNA metabolism, RNA helicases are the largest class of enzymes, and many of these proteins have emerged as central players in the regulation of gene expression at the RNA level. This volume of Methods in Enzymology aims to provide a reference for the diverse, powerful tools used to analyze RNA helicases. The contributions in this volume cover the broad scope of methods in the research on these enzymes. Several chapters describe quantitative biophysical and biochemical approaches to study molecular mechanisms and conformational changes of RNA helicases. Further chapters cover structural analysis, examination of co-factor effects on several representative examples, and the analysis of cellular functions of select enzymes. Two chapters outline approaches to the analysis of inhibitors that target RNA helicases. Zusammenfassung Covers the broad scope of methods in the research on RNA helicases enzymes. This title features chapters that describe quantitative biophysical and biochemical approaches to study molecular mechanisms and conformational changes of RNA helicases. It covers structural analysis and examination of co-factor effects on several representative examples. Inhaltsverzeichnis Analysis of Duplex Unwinding by RNA Helicases Using Stopped Flow Fluorescence Spectroscopy Analyzing ATP Utilization by DEAD-box RNA Helicases Using Kinetic and Equilibrium Methods Oxygen Isotopic Exchange Probes of ATP Hydrolysis by RNA Helicases Conformational Changes of DEAD-box Helicases Monitored by Single Molecule Fluorescence Resonance Energy Transfer RNA Catalysis as a Probe for Chaperone Activity of DEAD-box Helicases Molecular Mechanics of RNA Translocases Analysis of Helicase-RNA Interactions Using Nucleotide Analog Interference Mapping Crystallization and X-ray Structure Determination of an RNA-dependent Hexameric Helicase Structural Analysis of RNA Helicases with Small-angle X-ray Scattering Analysis of Co-factor Effects on RNA Helicases Analysis of DEAD-box Proteins in mRNA Export Biochemical Characterization of the RNA Helicase UPF1 Involved in Nonsense-mediated mRNA Decay Identification of RNA Helicase Target Sites by UV Crosslinking and Analysis of cDNA (CRAC) In Vivo Approaches to Dissecting the Function of RNA Helicases in Eukaryotic Ribosome Assembly Analysis of RNA Helicases in P-bodies and Stress Granules DEAD box RNA Helicases as Transcription Co-factors DEAD -box RNA Helicases in Gram-positive RNA-decay RNA Helicases in Cyanobacteria: Biochemical and Molecular Approaches Determination of Host RNA Helicases Activity in Viral Replication Inhibitors of Translation Targeting Eukaryotic Translation Initiation Factor 4A Identification and Analysis of Inhibitors Targeting the Hepatitis C Virus NS3 Helicase ...

Zusammenfassung
Covers the broad scope of methods in the research on RNA helicases enzymes. This title features chapters that describe quantitative biophysical and biochemical approaches to study molecular mechanisms and conformational changes of RNA helicases. It covers structural analysis and examination of co-factor effects on several representative examples.

Inhalt

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1. Analysis of Duplex Unwinding by RNA Helicases Using Stopped Flow Fluorescence Spectroscopy
2. Analyzing ATP Utilization by DEAD-box RNA Helicases Using Kinetic and Equilibrium Methods** **
3. Oxygen Isotopic Exchange Probes of ATP Hydrolysis by RNA Helicases** **
4. Conformational Changes of DEAD-box Helicases Monitored by Single Molecule Fluorescence Resonance Energy Transfer** **
5. RNA Catalysis as a Probe for Chaperone Activity of DEAD-box Helicases** **
6. Molecular Mechanics of RNA Translocases** **
7. Analysis of Helicase-RNA Interactions Using Nucleotide Analog Interference Mapping**
8. Crystallization and X-ray Structure Determination of an RNA-dependent Hexameric Helicase **
9. Structural Analysis of RNA Helicases with Small-angle X-ray Scattering** **
10. Analysis of Co-factor Effects on RNA Helicases** **
11. Analysis of DEAD-box Proteins in mRNA Export** **
12. Biochemical Characterization of the RNA Helicase UPF1 Involved in Nonsense-mediated mRNA Decay** **
13. Identification of RNA Helicase Target Sites by UV Crosslinking and Analysis of cDNA (CRAC)** **
14. In Vivo Approaches to Dissecting the Function of RNA Helicases in Eukaryotic Ribosome Assembly
15. Analysis of RNA Helicases in P-bodies and Stress Granules** **
16. DEAD box RNA Helicases as Transcription Co-factors** **
17. DEAD*-box RNA Helicases in Gram-positive RNA-decay*
18. RNA Helicases in Cyanobacteria: Biochemical and Molecular Approaches** **
19. Determination of Host RNA Helicases Activity in Viral Replication** **
20. Inhibitors of Translation Targeting Eukaryotic Translation Initiation Factor 4A
21. *Identification and Analysis of Inhibitors Targeting the Hepatitis C Virus NS3 Helicase * **