A Bacterial Transgene for Catalase Protects Translation of D1 Protein

Abstract The pBI101-katE binary vector harboring katE gene derived from Escherichia coli and driven by the promoter of the Rubisco small subunit gene of tomato, rbcS3C, was isolated and molecular characterized then transformed into competent cells using the heat chock method. The binary vector was transformed into competent Agrobacterium tumefaciens cells by two ways; the first way via the tri-parental mating method, and the second one by the electroporation method. The pBI101-katE construct was introduced into a dwarf tomato via Agrobacterium tumefaciens-mediated transformation. And also the construct was introduced into a tobacco by biolistic bombardment.Putative transgenic plants were regenerated and further screened by using PCR. PCR analysis of T1 plants from the independent calli revealed the presence of katE and nptII genes.

Autorentext

Khaled AlTaweel, Wydziä Farmacji, Uniwersytet Manitoba, Winnipeg, Manitoba, Kanada Doktor in ynierii genetycznej i genomiki funkcjonalnej, Uniwersytet Prefektury Osaka, Japonia. Pracownik naukowy w dziedzinie ekspresji genów czynnika wzrostu nerwów. Wydziä Farmacji, Uniwersytet Manitoba, Kanada.