Acute Myeloid Leukemia

This volume describes up-to-date techniques used in understanding the molecular biology of acute myeloid leukemia (AML) and addressing advances in diagnosis, classification, prognostication, and therapeutic strategies to potentially impact overall patient survival. The chapters in this book cover topics such as: cytochemical staining, single-cell mass cytometry of AML and Leukemia stem/progenitor cells, microsphere-based assessment of DNA methylation for AML prognosis, a zebrafish model for evaluating the function of human leukemic gene IDH1 and its mutation, and the isolation of biologically active exosomes from plasma of patients with cancer. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.

Cutting-edge and practical, Acute Myeloid Leukemia: Methods and Protocols is a valuable resource for scientists and researchers to further their studies and advancements in the field of AML.

Includes cutting-edge methods and protocols Provides step-by-step detail essential for reproducible results Contains key notes and implementation advice from the experts

Inhalt

Molecular Malfeasance Mediating Myeloid Malignancies: The Genetics of Acute Myeloid Leukemia.- Cytochemical Staining.- Immunohistochemistry in Acute Myeloid Leukemia.- Immunophenotyping by Multiparameter Flow Cytometry.- Single-Cell Mass Cytometry of Acute Myeloid Leukemia and Leukemia Stem/Progenitor Cells.- Design and Application of Multiplex-PCR-Seq for the Detection of Somatic Mutations Associated with Myeloid Malignancies.- AMLProfiler: A Diagnostic and Prognostic Microarray for Acute Myeloid Leukemia.- Microsphere-Based Assessment of DNA Methylation for AML Prognosis.- Genome-Wide Analysis of DNA Methylation in Hematopoietic Cells: DNA Methylation Analysis by WGBS.- Detection and Quantification of Acute Myeloid Leukemia-Associated Fusion Transcripts.- Targeted Next-Generation Sequencing of Acute Leukemia.- Bioinformatics Data Analysis of Next-Generation Sequencing Data from Heterogeneous Tumor Samples.- A Zebrafish Model for Evaluating the Function of Human Leukemic Gene IDH1 and its Mutation.- Measurement of Oncometabolites D-2-Hydroxyglutaric Acid and L-2-Hydroxyglutaric Acid.- Nanofluidic Allele-Specific Digital PCR Method for Quantifying IDH1 and IDH2 Mutation Burden in Acute Myeloid Leukaemia.- Isolation of Biologically Active Exosomes from Plasma of Patients with Cancer.- Generating and Expanding Autologous Chimeric Antigen Receptor T Cells from Patients with Acute Myeloid Leukemia.