Advanced NMR Techniques for Biomarkers Identification

Nuclear Magnetic Resonance spectroscopy has emerged as a key tool for understanding metabolic processes in living systems. Recently, a new approach to elucidate metabolism and its mechanisms appeared to the scientific community scene. It is metabonomics: an analysis based on very few assumptions on the biochemical processes that occur in living systems, mainly investigated by advanced spectroscopic techniques including mass spectrometry and NMR spectroscopy. The development of new spectroscopic tools for high thoughput analysis of selected biochemical pathways is crucial for metabolome investigations. The purpose of the present work was to explore the recent NMR improvements by applying and developing new metabolomic strategies for biomarkers discovery, including NMR data handling, peaks quantification and fast data acquisition. This book is integrally extracted from my PhD thesis.

Autorentext

Debora Paris received the Graduation in Physics cum laude fromthe University of Napoli "Federico II" in the 2006 and the Ph.Din Chemistry from the same University in 2009. She has aPostdoctoral fellow at CNR-ICB Institute of BiomolecularChemistry of Pozzuoli (Italy). Since 2004 she has been working onNMR spectroscopy applied to metabolomics.