Advancing Development of Synthetic Gene Regulators

Nominated by Kyoto University as an outstanding Ph.D. thesis
Reviews multitasking applications of next-generation sequencing (NGS) technologies for chemical biologists
Describes low-cost Bind-n-Seq and small molecules based genomic pull-down development, identification of high-affinity binding sites for PIP conjugates using high-throughput sequencing, and the binding motif-guided redesign of PIPs

Nominated by Kyoto University as an outstanding Ph.D. thesis Reviews multitasking applications of next-generation sequencing (NGS) technologies for chemical biologists Describes low-cost Bind-n-Seq and small molecules based genomic pull-down development, identification of high-affinity binding sites for PIP conjugates using high-throughput sequencing, and the binding motif-guided redesign of PIPs Includes supplementary material: sn.pub/extras

Inhalt

Overview of Next-Generation Sequencing Technologies and its application in Chemical Biology.- Next Generation Sequencing Studies Guide the Design of Pyrrole-Imidazole Polyamides with Improved Binding Specificity by the Addition of -alanine.- Genome-Wide Assessment of the Binding Effects of Artificial Transcriptional Activators by Utilizing the Power of High-Throughput Sequencing.- Deciphering the genomic targets of alkylating polyamide conjugates using high-throughput sequencing. <p