Analytical Ultracentrifugation: Volume 562

*Analytical Ultracentrifugation, the latest volume in Methods in Enzymology,* focuses on analytical ultracentrifugation. The scope of this technique has greatly expanded in recent years due to advances in instrumentation, algorithms and software.

This volume describes the latest innovations in the field and in the applications of analytical ultracentrifugation in the analysis of macromolecules, macromolecular assemblies, and biopharmaceuticals.

Autorentext
Dr. Cole received a Sc.B. in Biochemistry from Brown University. He obtained his Ph.D. in Biophysical Chemistry at UC Berkeley where he investigated the mechanism of photosynthetic oxygen evolution. He carried out postdoctoral research at Stanford University on the structure and function of metalloenzymes that interact with oxygen. In 1991, he took a position at Merck Research Laboratories in antiviral drug discovery. Dr. Cole applied analytical ultracentrifugation and related biophysical techniques to probe macromolecular interactions that regulate replication of HIV, Influenza and Hepatitis C virus. In 2001, he to return to academia and moved to the Department of Molecular and Cell Biology at the University of Connecticut, where he is professor of structural biology, biophysics and biochemistry. His research group uses biophysical approaches to characterize macromolecular interactions that regulate important biological processes. A major focus is the innate immunity pathway for defense against viral infection. In an NIH-supported research program, his group has defined the mechanism for activation of protein kinase R by RNA. He directs the National Analytical Ultracentrifugation Facility and holds a joint appointment in the department of Chemistry at the University of Connecticut. He is a member of the editorial board of Biophysical Journal. He is the author of 84 publications and reviews.

Klappentext

Analytical Ultracentrifugation, the latest volume in Methods in Enzymology, focuses on analytical ultracentrifugation. The scope of this technique has greatly expanded in recent years due to advances in instrumentation, algorithms and software.

This volume describes the latest innovations in the field and in the applications of analytical ultracentrifugation in the analysis of macromolecules, macromolecular assemblies, and biopharmaceuticals.

Inhalt

1. Next Generation AUC adds a Spectral Dimension: Development of Multiwavelength Detectors for the Analytical Ultracentrifuge
   Joseph Pearson, Frank Krause, Dirk Haffke, Borries Demeler, Kristian Schilling and Helmut Cölfen
   
   2. Next Generation AUC: Analysis of Multi-Wavelength Analytical Ultracentrifugation Data
      Gary E. Gorbet, Joseph Z. Pearson, Aysha K. Demeler, Helmut Cölfen and Borries Demeler
   3. Sedimentation Velocity: A Classical Perspective
      John J. Correia and Walter F. Stafford
   4. Hydrodynamic Modeling and its Application in AUC
      Mattia Rocco and Olwyn Byron
   5. Calculations and Publication-Quality Illustrations for Analytical Ultracentrifugation Data
      Chad A. Brautigam
   6. Sedimentation Equilibrium Analysis of Clpb Self-Association in Diluted and Crowded Solutions.
      Carlos Alfonso, Urko del Castillo, Ianire Martín, Arturo Muga and Germán Rivas
   7. Analysis of Linked Equilibria
      JiaBei Lin and Aaron L. Lucius
   8. Elucidating Complicated Assembling Systems in Biology Using Size-And-Shape Analysis of Sedimentation Velocity Data
      Catherine T. Chaton and Andrew B. Herr
   9. Quaternary Structure Analyses of an Essential Oligomeric Enzyme
      Tatiana P. Soares da Costa, Janni B. Christensen, Sebastien Desbois, Shane E. Gordon, Ruchi Gupta, Campbell J. Hogan, Tao G. Nelson, Matthew T. Downton, Chamodi K. Gardhi, Belinda M. Abbott, John Wagner, Santosh Panjikar and Matthew A. Perugini
   10. Characterization of Intrinsically Disordered Proteins by Analytical Ultracentrifugation
       David J. Scott and Donald J. Winzor
   11. Sedimentation Velocity Analysis of the Size Distribution of Amyloid Oligomers and Fibrils
       Yee-Foong Mok, Geoffrey J. Howlett and Michael D.W. Griffin
   12. AUC and Small Angle Scattering for Membrane Proteins
       Aline Le Roy, Kai Wang, Béatrice Schaack, Peter Schuck, Cécile Breyton and Christine Ebel
   13. Hydrodynamic Models of G-quadruplex Structures
       Jonathan B. Chaires, William L. Dean, Huy Le and John O. Trent
   14. Analytical Ultracentrifugation as a Tool to Study Non-specific Protein-DNA Interactions
       Teng-Chieh Yang, Carlos Enrique Catalano and Nasib Karl Maluf
   15. Characterization of Homogeneous, Cooperative Protein-DNA Clusters by Sedimentation Equilibrium Analytical Ultracentrifugation and Atomic Force Microscopy
       Ingrid Tessmer and Michael G. Fried
   16. Sedimentation Velocity Analysis of Large Oligomeric Chromatin Complexes Using Interference Detection.
       Hansen and Jeffrey
   17. Methods in Enzymology - Analytical Ultracentrifugation
       David L Bain, Rolando W De Angelis, Keith D Connaghan, Qin Yang, Gregory D Degala and James R Lambert
   18. Ultracentrifuge Methods for the Analysis of Polysaccharides, Glycoconjugates and Lignins
       Stephen E. Harding, Gary G. Adams, Fahad Almutairi, Qushmua Alzahrani, Tayyibe Erten, M. Samil Kök and Richard B. Gillis
   19. Analytical Ultracentrifugation and its Role in Development and Research of Therapeutical Proteins.
       Jun Liu, Sandeep Yadav, James Andya, Barthélemy Demeule and Steven J. Shire
   20. Guidance to Achieve Accurate Aggregate Quantitation in Biopharmaceuticals by SV-AUC
       Kelly K. Arthur, Brent S. Kendrick and John P. Gabrielson
   21. Protein Assembly in Serum and the Differences from Assembly in Buffer.
       John J. Hill and Thomas M. Laue