Antibiotic Resistance Protocols

This third edition provides a wide range of different technologies, ranging from conventional growth basic techniques, application of molecular biology, development of resistance mutations, and diagnosis and monitoring treatment response. New and updated chapters cover techniques from the microscopic scale to whole animal models. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.

Authoritative and practical, Antibiotic Resistance Protocols, Third Edition aims to ensure successful results in the further study of this vital field.

Includes cutting-edge methods and protocols Provides step-by-step detail essential for reproducible results Contains key notes and implementation advice from the experts

Inhalt

Methods for Measuring the Production of Quorum Sensing Signal Molecules.- Construction and Use of Staphylococcus aureus Strains to Study Within-Host Infection Dynamics.- Method for Detecting and Studying Genome-wide Mutations in Single Living Cells in Real-time.- Detecting Phenotypically Resistant Mycobacterium tuberculosis using Wavelength Modulated Raman Spectroscopy.- A Flow Cytometry Method for Assessing M. tuberculosis Responses to Antibiotics Charlotte.- Application of Continuous Culture for Assessing Antibiotic Activity against Mycobacterium tuberculosis .- Real-Time Digital Bright Field Technology for Rapid Antibiotic Susceptibility Testing.- Enhanced Methodologies for Detecting Phenotypic Resistance in Mycobacteria.- Methods to Determine Mutational Trajectories after Experimental Evolution of Antibiotic Resistance.- Selection of ESBL-producing E. coli in a Mouse Intestinal Colonization Model.- Transcriptional Profiling Mycobacterium tuberculosis from Patient Sputa.- Direct Agarose Gel Probing, Pulsed Field Gel Electrophoresis, Plasmid detection, Genomic Location, and Resistance Gene Mapping.- Using RT qPCR for Quantifying Mycobacteria marinum from In-vitro and In-vivo Samples.- Use of Larval Zebrafish Model to Study Within-Host Infection Dynamics.- A Method to Evaluate Persistent Bacteria in vitro and in the Cornell Model of Tuberculosis.