Antigens from Leishmania Amastigotes, protect Human against Leishmania

In the endemic "Valle Arriba" neighborhood, located in Caracas, Venezuela, with leishmaniasis morbidity 24.76 o/oo, an amastigotes vaccine with four Leishmania species was applied for protection against leishmaniasis in 3,191 people with a follow up of three years and also in hyperendemic "El Ingenio" with morbidity of 164.04 o/oo. The vaccine contains 8-10 proteins after treatment amastigotes with TLCK and NP40. Before the vaccine both vaccinated and control groups had 24% of positive volunteers to Delayed Type Hypersensitivity intradermic reaction with vaccine antigens. One month after three vaccines, 95.67% of vaccinees previously DTH negative converted to a positive DTH. The morbidity rate was 7.94 o/oo in the vaccinated group, and 56.17 in the control-unvaccinated group, an incidence 7.07 times bigger after three years of the field trial. In "La Planta" hyperendemic zone 41.78% were DTH positive before vaccination, with 122 cases from 1972-1994 and highest morbidity (205.23 o/oo) in 0-5 years old, which points out to intra-domiciliary transmission of the disease . ELISA and immunoblottings were equal before and after vaccination, the vaccine induced cellular immunity.

Autorentext

Jose Antonio O'Daly Carbonell. Amerikanischer Staatsbürger. Geburtsland: Caracas, Venezuela. Sekundarschule: La-Salle-Gymnasium Caracas. Universität: Zentraluniversität von Venezuela Caracas: MD 1958-1964 DMSc 1965-1968. Johns Hopkins Universität PhD 1967-1071. Veröffentlichungen Internationale Fachzeitschriften mit Peer-Review 117 Eingeladen von den Herausgebern 18. Insgesamt 135.

Klappentext

In the endemic "Valle Arriba" neighborhood, located in Caracas, Venezuela, with leishmaniasis morbidity 24.76 o/oo, an amastigotes vaccine with four Leishmania species was applied for protection against leishmaniasis in 3,191 people with a follow up of three years and also in hyperendemic "El Ingenio" with morbidity of 164.04 o/oo. The vaccine contains 8-10 proteins after treatment amastigotes with TLCK and NP40. Before the vaccine both vaccinated and control groups had 24% of positive volunteers to Delayed Type Hypersensitivity intradermic reaction with vaccine antigens. One month after three vaccines, 95.67% of vaccinees previously DTH negative converted to a positive DTH. The morbidity rate was 7.94 o/oo in the vaccinated group, and 56.17 in the control-unvaccinated group, an incidence 7.07 times bigger after three years of the field trial. In "La Planta" hyperendemic zone 41.78% were DTH positive before vaccination, with 122 cases from 1972-1994 and highest morbidity (205.23 o/oo) in 0-5 years old, which points out to intra-domiciliary transmission of the disease . ELISA and immunoblottings were equal before and after vaccination, the vaccine induced cellular immunity.