Artificial Assemblies with Cooperative DNA Recognition

This book presents three types of synthetically cooperative DNA recognizing assemblies, in order to advance the development of programmable DNA-binding pyrroleimidazole polyamides (PIPs).

PIPs represent the best-characterized class of small molecule DNA binders that can be modified to bind with any predetermined DNA sequence and regulate gene expression patterns in a transgene-free and cost-effective manner. PIPs are characterized by their small molecular size, high binding affinity, programmability, sequence selectivity, and moderate cell permeability. In recent years, there have been numerous novel studies on the applications of these biological tools; this research is thoroughly reviewed in the first chapter.

There are several critical issues, however, that impede the further broad study of PIPs, which greatly concern the author. For instance, the short PIP version has an excessively hi^10 bp; this significantly decreases cell permeability. Moreover, the conventional binding strategy for PIP design cannot apply to flexible DNA bindingfor example, the DNA-binding mode of a transcription factor pair.

In this book, the author describes the development of three kinds of cooperative DNA-binding systems that help resolve the current highly problematic issues concerning PIPs. These three systems offer a range of significant advantages, such as favorable sequence selectivity, long recognition sequence, higher binding affinity, and a flexible gap distance.

Released at a critical juncture in the application of PIPs, this book will greatly facilitate their use as therapeutic drugs in the treatment of cancer and hereditary diseases, and in regenerative medicine.

Nominated as an outstanding PhD thesis by Kyoto University Is the first-ever report on three elegant designs of cooperative DNA-binding systems Highlights the most important aspects of programmable DNA-binding pyrroleimidazole polyamides for quick learning

Inhalt

1. Synthetic transcription factors (Syn-TFs): design, progress and perspectives.- 2. Pip-HoGu, an artificial assembly with cooperative DNA recognition capable of mimicking transcription factor pairs.- 3. Orthogonal PNA dimerization domains empower DNA binders with cooperativity and versatility mimicking that of the transcription factor pairs.- 4. Advanced DNA binding system mimicking the cooperative function of transcription factor pairs precisely recruits the epigenetic modifiers to the DNA repeat binding sites.