Avidin-Biotin Interactions

This book is specifically about the application of the extremely powerful interaction between the protein avidin or its homologues and the vitamin biotin and some of its homologues. With excellent descriptions of laboratory protocols written by expert researchers, this volume is equally perfect for the student or the professional laboratory scientist.

Excellent descriptions of laboratory protocols written by expert researchers Equally perfect for the student or the professional laboratory scientist

Inhalt
Preparation of Avidin Conjugates.- Coupling of Antibodies With Biotin.- Optimization of Detection and Quantification of Proteins on Membranes in Very High and Very Low Abundance Using Avidin and Streptavidin.- Use of the Avidin (Imino)Biotin System as a General Approach to Affinity Precipitation.- Capturing Biotinylated Proteins and Peptides by Avidin Functional Affinity Electrophoresis.- Functionality Screen of Streptavidin Mutants by Non-Denaturing SDSPAGE Using Biotin-4-Fluorescein.- Application of Biotin-4-Fluorescein in Homogeneous Fluorescence Assays for Avidin, Streptavidin, and Biotin or Biotin Derivatives.- Quantitative Recovery of Biotinylated Proteins from Streptavidin-Based Affinity Chromatography Resins.- High-Yield Production and Purification of Recombinant T7-Tag Mature Streptavidin in Glucose-Stressed E. coli.- Endogenous Biotin in Rat Brain.- Pseudo-Immunolabelling With the AvidinBiotinPeroxidase Complex due to the Presence of Endogenous Biotin in the Retina.- Use of Synthetic Peptides for Identifying Biotinylation Sites in Human Histones.- Detection and Quantitation of the Activity of DNA Methyltransferases Using a Biotin/Avidin Microplate Assay.- Anti-Biotin Antibodies Offer Superior Organelle-Specific Labelling of Mitochondria Over Avidin or Streptavidin.- Mammalian Lectin as Tool in Glycochemistry and Histochemistry With Relevance for Diagnostic Procedure.- A Multiplex Approach to Isotyping Antigen-Specific Antibodies Using Biotinylated Antigen/Streptavidin-Phycoerythrin.- BiotinProtein Bond: Instability and Structural Modification to Provide Stability for In Vivo Applications.