Bacterial Vaccines

The importance of vaccines to combat bacterial diseases cannot be overstated. Methods used in the development and testing of these vaccines are evolving rapidly as a direct consequence of the availability of advanced technologies. This volume will cover methods developed in the last decade, the usage of which are enabling the development of cheaper, cost-effective and structurally stable vaccines for global use. Chapters cover in silico analytical methods such as reverse vaccinology and machine learning; low-energy electron irradiation for the generation of inactivated bacterial vaccines; methods for assessment of OMV/GMMA quality and stability; and controlled human infection models. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.

Cutting-edge and comprehensive, Bacterial Vaccines: Methods and Protocols is a valuable resource for novice and expert researchers interested in learning more about this important and constantly evolving field.

Includes cutting-edge methods and protocols Provides step-by-step detail essential for reproducible results Contains key notes and implementation advice from the experts

Inhalt
Vaccine Design by Reverse Vaccinology and Machine Learning.- Application of Reverse Vaccinology and Immunoinformatic Strategies for the Identification of Vaccine Candidates against Shigella flexneri.- Purification of Prospective Vaccine Antigens from Gram-Positive Pathogens by Immunoprecipitation.- Rapid Surface Shaving for Proteomic Identification of Novel Surface Antigens for Vaccine Development.- Two-Dimensional Electrophoresis Coupled with Western Blot as a Method to Detect Potential Neutralizing Antibody Targets from Gram-Negative Intracellular Bacteria.- Panproteome Analysis of the Human Antibody Response to Bacterial Vaccines and Challenge.- Low-Energy Electron Irradiation (LEEI) for the Generation of Inactivated Bacterial Vaccines.- Design and Production of Hybrid Antigens for Targeting Integral Outer Membrane Proteins in Gram-Negative Bacteria.- Preparation of Trimethyl Chitosan-Based Polyelectrolyte Complexes for Peptide Subunit VaccineDelivery.- Multiepitope Fusion Antigen - MEFA, An Epitope- and Structure-Based Vaccinology Platform for Multivalent Vaccine Development.- Production, Isolation, and Characterization of Bioengineered Bacterial Extracellular Membrane Vesicles Derived from Bacteroides thetaiotaomicron and Their Use in Vaccine Development.- Membrane Vesicles Produced by Shewanella vesiculosa HM13 as a Prospective Platform for Secretory Production of Heterologous Proteins at Low Temperatures.- Glycine Induction Method: Effective Production of Immunoactive Bacterial Membrane Vesicles with Low Endotoxin Content.- Methods for Assessment of OMV/GMMA Quality and Stability.- Production of Vaccines using Biological Conjugation.- Immunological Assessment of Lung Responses to Inhalational Lipoprotein Vaccines against Bacterial Pathogens.- Determination of Maternal and Infant Immune Responses to Pertussis Vaccination in Pregnancy.- Generation of a Universal Human Complement Source by Large-Scale Depletion of IgG and IgM from Pooled Human Plasma.- Assessment of Serum Bactericidal and Opsonophagocytic Activity of Antibodies to Gonococcal Vaccine Targets.- Opsonophagocytic Killing Assay to Measure Anti-Group A Streptococcus Antibody Functionality in Human Serum.- Neisseria lactamica Controlled Human Infection Model.- Analyzing Macrophage Infection at the Organ Level.- Multi-Color Flow Cytometry and High-Dimensional Data Analysis to Probe Complex Questions in Vaccinology.