Baculovirus and Insect Cell Expression Protocols

Baculovirus Expression Protocols is a detailed guide for using the baculovirus expression vector system (BEVS) and/or insect cells to produce recombinant proteins. Procedures for the development and production of baculovirus insecticides at both laboratory and large scale are described in the kind of step-by-step format that the Methods in Molecular Biology™ series helped standardize. This book is a one-stop source for information on baculoviruses, and includes sections on using modified baculoviruses to express genes in mammalian cells, using Drosophila cell lines, isolating new cell lines, and developing serum-free medium. Specific applications, including using baculovirus and insect cell system to study apoptosis, are also discussed. This updated and expanded edition of Baculovirus Expression Protocols provides a perfect lab manual for biochemists, molecular biologists, biochemical engineers, and others for using the BEVS or insect cell lines to produce recombinant proteins.

Includes useful tips for baculovirus research and detailed procedures for quantifying cell metabolic behavior Lists all available insect cell lines and provides methods for isolating new cell lines Numerous methods and protocols to inspire new techniques and applications for baculovirus and insect cell culture

Klappentext

Baculovirus Expression Protocols, Second Edition, provides the detailed steps required to perform the techniques involved with the use of baculoviruses and insect cell culture and discusses problems that may be encountered. This newly-revised and updated edition provides a cutting edge step-by-step guide for biochemists, molecular biologists, biochemical engineers and others using the BEVS and/or insect cells for producing recombinant proteins. Furthermore, Baculovirus Expression Protocols, Second Edition, provides assistance to scientists and engineers interested in developing and producing baculovirus insecticides. Authors list all available insect cell lines and provide methods for isolating new cell lines. The procedures involved in producing products, both at laboratory scale and large scale, are discussed, as well as the production in insect larvae. It is hoped that this volume will not only aid the user in successfully completing the tasks described herein, but will also stimulate the development of improved techniques and new applications of baculoviruses and insect cell culture. Timely and thorough, this volume will become an essential reference for anyone using baculoviruses and insect cell culture.

Inhalt
Useful Tips, Widely Used Techniques, and Quantifying Cell Metabolic Behavior.- Baculovirus Molecular Biology/Development of Recombinant Baculoviruses.- to Baculovirus Molecular Biology.- Baculovirus Transfer Vectors.- Recombinant Baculovirus Isolation.- Gene Expression in Mammalian Cells Using BacMam, a Modified Baculovirus System.- Insect Cell Culture.- Available Lepidopteran Insect Cell Lines.- Lepidopteran Insect Cell Line Isolation from Insect Tissue.- Development of Serum-Free Media for Lepidopteran Insect Cell Lines.- Routine Maintenance and Storage of Lepidopteran Insect Cell Lines and Baculoviruses.- Protein Production with Recombinant Baculoviruses.- Small-Scale Protein Production With the Baculovirus Expression Vector System.- Recombinant Protein Production in Large-Scale Agitated Bioreactors Using the Baculovirus Expression Vector System.- Production of Baculovirus-Expressed Recombinant Proteins in Wave Bioreactors.- Protein Production With Recombinant Baculoviruses in Lepidopteran Larvae.- Virus-like Particle and Viral Vector Production Using the Baculovirus Expression Vector System/Insect Cell System.- Recombinant Protein Production with Transformed Insect Cells.- Transforming Lepidopteran Insect Cells for Continuous Recombinant Protein Expression.- Transformation of Drosophila Cell Lines.- Transforming Lepidopteran Insect Cells for Improved Protein Processing.- Baculovirus Development and Production for Use as Insecticides.- to the Use of Baculoviruses as Biological Insecticides.- Baculovirus Insecticide Production in Insect Larvae.- Evaluation of the Insecticidal Efficacy of Wild-Type and Recombinant Baculoviruses.- Miscellaneous Techniques and Applications of the Baculovirus/Insect Cell System.- Monitoring and Visualization of Baculovirus Infection UsingGreen Fluorescent Protein Strategy.- Alternative Bioreactor Strategy for Probing Infection and Production.- Methods for Gene Silencing With RNAi.- Using the Baculovirus/Insect Cell System to Study Apoptosis.- Generation of Envelope-Modified Baculoviruses for Gene Delivery Into Mammalian Cells.