Basic Life Science Methods

Basic Life Science Methods: A Laboratory Manual for Students and Researchers presents forty of the most executed life science assays. The authors use a consistent structure to cover the preparation, execution and analysis of data from each method. Assays include estimation of cholesterol fractions, C-Reactive Protein, Genomic DNA isolation, Agarose Gel Electrophoresis, RT-PCR, DNA solution preparation, how to design primers, and enzyme-linked immunosorbent assay (ELISA). This book provides a complete reference containing step-by-step instructions on how to run life science assays. Laboratory staff can also benefit of the book as a training resource.

Autorentext

Dr. Javeed Ahmad Tantray's current research focus is on Human cardiovascular diseases obtained his Ph. D from Osmania University in Hyderabad, India. Dr. Tantray has done Post Doc studies with the Department of Zoology, University of Kashmir, receiving funding from the UGC-CSIR. He published many National and International peer-reviewed research articles. Dr. Tantray has published in high impact factor journals such as the International Journal of Cardiology, and Saudi journal of biological science. He has guided 50, M.Sc / and graduate students projects. He has received Junior scientist awards both at national and international level. He received the Best Abstract Award at the 4th Asia Pacific CardioMetabolic Syndrome Congress held by the Korean Society of CardioMetabolic Syndrome,with an award amount of USD 500. Dr. Tantray has organized a national seminar on "Cancer Bio-markers" and a one-day national workshop on "Assembly and usage of Foldscope?. Dr. Tantray is currently an Assistant Professor in the Department of Zoology, School of life sciences central university of Kashmir (J&K).

Inhalt

1. Calculations of Molarity and Normality
   
   2. Glassware washing and sterilization techniques
   3. pH Meter: Its use and calibration
   4. Principle and working of Autoclave
   5. Principle and working of Centrifuge
   6. Principles and working of Laminar
   7. Estimation of Total cholesterol (Flegg 1972)
   8. Estimation of Creatinine
   9. Estimation of blood Urea
   10. Estimation of blood glucose By GOD - POD method
   11. Determination of Albumin, and Globulin in Plasma
   12. Quantitative determination of Homocysteine
   13. Estimation of serum bilirubin
   14. Estimation of diagnostic enzyme ALT
   15. Estimation of diagnostic enzyme AST
   16. To Estimate Protein by Lowry's Method
   17. Estimation of Reducing sugar by using Dinitro Salicylic Acid (DNS) Method
   18. To perform quantitative determination of total protein in Serum/plasma
   19. Enzyme Activity Using Starch assay
   20. To Estimate Protein by Bradford's Assay
   21. To Identify a Protein Sample Using SDS-PAGE
   22. Genomic DNA isolation from whole blood
   23. PCR for gene amplification
   24. Agarose Gel Electrophoresis (AGE)
   25. RNA Isolation from Human tissue
   26. Real-time polymerase chain reaction (RT-qPCR)
   27. Preparation of competent cells by CaCl2 treatment
   28. RNA Isolation from Plant tissues
   29. DNA isolation from the bacterial culture broth
   30. DNA isolation from fungal Mycelium
   31. Quantification of the DNA using spectrophotometer
   32. Formaldehyde gel electrophoresis of the isolated RNA sample
   33. Paper chromatography of different amino acid
   34. Separation of plants pigments using Column Chromatography
   35. Separation of plant pigment by Thin Layer Chromatography
   36. Chromatin Immunoprecipitation (ChIP) Protocol
   37. Types of Media
   38. Preparation of Nutrient Agar Media
   39. Serial Dilution of the Bacterial Cells
   40. Spread Plate Method of The Bacterial Cells
   41. Streak Plate Method
   42. Identified Bacterial Colony in Culture Broth
   43. Pour plate method for bacterial colony counting
   44. Gram Staining of Bacteria
   45. Casein hydrolysis test
   46. Antibiotic resistance test by Agar Well Diffusion Method