Basic Neurobiology Techniques

This volume explores the latest techniques and improved methods used to study important neurobiological and associated neuropathological conditions. Chapters cover topics such as the application of semiconductor quantum dots as photostable fluorophores used for labeling and tracking; GCaMP6 transcranial imaging to monitor neural activity; the patch-clamping technique allowing simultaneous monitoring of membrane currents and optical signals; and quantifying behavioral dysfunction caused by traumatic brain injury (TBI). In Neuromethods series style, chapters include the kind of detail and key advice from the specialists needed to get successful results in your laboratory.

Cutting-edge and practical, Basic Neurobiology Techniques is a valuable resource for novel and expert researchers interested in expanding their knowledge of this developing field.

Includes cutting-edge methods and protocols Provides step-by-step detail essential for reproducible results Contains key notes and implementation advice from the experts

Inhalt
Semiconductor Quantum Dots for Visualization and Sensing in Neuronal Cell Systems.- Injection of Adeno-Associated Virus Containing Optogenetic and Chemogenetic Probes into the Neonatal Mouse Brain.- Guide to Transcranial Imaging of Sound-Evoked Activity in the Auditory Cortex of GCaMP6s Mice In Vivo.- A Novel Pre-Clinical Rat Model of Alzheimer's Disease.- Using Zebrafish to Study and Visualize Barriers of the CNS.- Photometric Patch Electrode to Simultaneously Measure Neural Electrical Activity and Optical Signal in the Brain Tissue.- Patch-Clamp Fluorometry and Its Applications to the Study of Ion Channels.- Modeling Early-Onset Post-Ischemic Seizures in Adult and Aging Mice.- Modeling Psychological Trauma in Rats.- Extending the Life-Span of Acute Neuronal Tissue for Imaging and Electrophysiological Studies.- Confocal Imaging of Fast Flash Photolysis of Caged Compounds in Cultured Neurons.- Analysis of the Functional Statesof an Astrocyte Syncytium.- Assessment of Behavioral Dysfunction following Experimental Traumatic Brain Injury (TBI).