Biochemical Pathways and Environmental Responses in Plants: Part A: Volume 676

Biochemical Pathways and Environmental Responses in Plants, Part A, Volume 676 in the Methods in Enzymology series highlights new advances in the field with this new volume presenting interesting chapters on topics such as Structure, function, and engineering of plant polyketide synthases, A sensitive LC-MS/MS assay for enzymatic characterization of methylthioalkylmalate synthase involved in glucosinolate side-chain elongation, Assaying formate-tetrahydrofolate ligase with monoglutamylated and polyglutamylated substrates using a fluorescence-HPLC based assay, An Approach to Nearest Neighbor Analysis of Pigmented Protein Complexes by Using Chemical Crosslinking in Combination with Mass Spectrometry, and much more.

Other chapters cover Biochemical characterization of plant aromatic aminotransferases, Functional Analysis of Phosphoethanolamine N-methyltransferase (PMT) in Plants and Parasites, A structure-guided computational screening approach for predicting plant enzyme-metabolite interactions, Plant metacaspase: an example of microcrystal structure determination and analysis, Biocatalytic system for comparative assessment of functional association of cytochrome P450 monooxygenases with their redox partners, Dirigent Protein Family Function and Structure, and more.

Autorentext
Prof. Jez received his B.S. in biochemistry from Penn State University (1992), a Ph.D. in biochemistry & molecular biophysics from the University of Pennsylvania (1998), and was an NIH-NRSA postdoctoral fellow at the Salk Institute for Biological Studies (1998-2001). After working as a research scientist at Kosan Biosciences (2001-2002), he started his research group at the Donald Danforth Plant Science Center and moved to the Department of Biology at Washington University in 2008. He has authored more than 160 papers and received a Presidential Early Career Award for Scientists and Engineers (2005), the Phytochemical Society of North America's Arthur Neish Young Investigator Award (2007), and a Fulbright Senior Specialist Award (2012) and was named as Howard Hughes Medical Institute Professor in 2014. Research in the Jez lab seeks to understand how environmental changes re-model biochemical pathways in plants at the molecular, cellular, and organism levels with the aim of engineering these systems to address agricultural and environmental problems. Current work in the lab employs a combination of structural biology, protein chemistry, and plant biology. He also launched the Biotech Explorers Program, which aims to introduce undergraduates to how teams in science can tackle real world problems using cross-disciplinary approaches.

Inhalt

1. Structure, function, and engineering of plant polyketide synthases
   Ikuro Abe
2. A sensitive LC-MS/MS assay for enzymatic characterization of methylthioalkylmalate?synthase involved in glucosinolate side-chain elongation
   N Bisht
3. Assaying formate-tetrahydrofolate ligase with monoglutamylated and polyglutamylated substrates using a fluorescence-HPLC based assay
   Sanja Roje
   4. An Approach to Nearest Neighbor Analysis of Pigmented Protein Complexes by Using Chemical Crosslinking in Combination with Mass Spectrometry
   Haijun Liu
4. Biochemical characterization of plant aromatic aminotransferases
   Hiroshi Maeda
5. Functional Analysis of Phosphoethanolamine N-methyltransferase (PMT) in Plants and Parasites
   Soon Goo Lee
6. A structure-guided computational screening approach for predicting plant enzyme-metabolite interactions
   Cynthia Holland
7. Plant metacaspase: an example of microcrystal structure determination and analysis
   Qun Liu
8. Biocatalytic system for comparative assessment of functional association of cytochrome P450 monooxygenases with their redox partners
   Chang-Jun Liu
   10. Dirigent Protein Family Function and Structure
   Norman G. Lewis
   11. Lignin/Lignan Biosynthesis Structure/Function
   Norman G. Lewis
9. Discovery and characterization of BAHD acylsugar acyltransferases
   Robert L. Last
10. Near-real time determination of BAHD acyl-coenzyme A transferase reaction rates and kinetic parameters using Ellman's reagent
    Michael Sullivan
11. Quantification of N-terminal protein acetyltransferase activity in vitro and in planta
    Markus Wirtz
12. Computational and biochemical methods to measure the activity of carboxysomes and protein organelles in vivo
    Jeffrey Cameron
    16. Analysis of Plant Alkaloid-Protein Interactions Using Differential Scanning Fluorimetry and Isothermal Titration Calorimetry
    Peter Facchini
    17. A metabolomics strategy for plant specialized metabolites using liquid chromatography-mass spectrometry
    Kazuki Saito and Tetsuya Mori
13. Isoprene measurements to assess the methylerythritol pathway
    Thomas D. Sharkey
    19. Analysis of isoprenoid alcohol phosphates by liquid chromatorgraphy-mass spectrometry
    Peter Dörmann and Katharina Gutbrod
14. Using targeted metabolomics to elucidate the indole auxin network in plants
    Jerry D. Cohen and Adrian D. Hegeman
    21. Metabolite fingerprinting: A powerful metabolomics approach for marker identification and functional gene annotation
    Ivo Feussner
    22. Ex vivo metabolomics, an hypothesis-free approach to identify native substrate(s) of orphan enzymes
    Kirstin Feussner
    23. Using stable isotopes and LC-MS for precursor of origin determination in plants
    Clint Chapple
15. Measurement of flux through sulfate assimilation using [35S]sulfate
    Stanislav Kopriva
    25. Assessment of the activity of sulfate uptake and assimilation in plants
    Rüdiger Hell
    26. Comparison of TLC, HPLC, and direct infusion mass spectrometry methods for the identification and quantification of diacylglycerol molecular species
    Philip Bates
    27. Plant sphingolipid analysis
    Jennifer E. Markham
16. Strategies to study the metabolic origins of plant specialized metabolites: the 1,4-naphthoquinones in Lithospermum erythrorhizon
    Joshua R. Widhalm
17. Controlled environments for highly reproducible cannabis 'omics' studies
    Bernd Markus Lange
18. Multi-platform approaches for the comprehensive analysis of aroma volatiles in cannabis
    Bernd Markus Lange
19. Systems and strategies for plant protein expression
    Thomas D. Niehaus
20. Programmable Chemical Actuator Control of Soluble and Membrane-Bound Enzymatic