Biochemical Pathways and Environmental Responses in Plants: Part B: Volume 680

Biochemical Pathways and Environmental Responses in Plants, Part B, Volume 682 in the Methods in Enzymology series, highlights advances in the field with this new volume presenting chapters on MIE 681/682: Biochemical pathways and environmental responses in plants, Structure, function, and engineering of plant polyketide synthases, A sensitive LC-MS/MS assay for enzymatic characterization of methylthioalkylmalate synthase involved in glucosinolate side-chain elongation, Assaying formate-tetrahydrofolate ligase with monoglutamylated and polyglutamylated substrates using a fluorescence-HPLC based assay, An Approach to Nearest Neighbor Analysis of Pigmented Protein Complexes by Using Chemical Crosslinking in Combination with Mass Spectrometry, Biochemical characterization of plant aromatic aminotransferases, and much more.

Other chapters focus on Functional Analysis of Phosphoethanolamine N-methyltransferase (PMT) in Plants and Parasites, A structure-guided computational screening approach for predicting plant enzyme-metabolite interactions, Plant metacaspase: an example of microcrystal structure determination and analysis, Biocatalytic system for comparative assessment of functional association of cytochrome P450 monooxygenases with their redox partners, Dirigent Protein Family Function and Structure, and more.

Autorentext
Prof. Jez received his B.S. in biochemistry from Penn State University (1992), a Ph.D. in biochemistry & molecular biophysics from the University of Pennsylvania (1998), and was an NIH-NRSA postdoctoral fellow at the Salk Institute for Biological Studies (1998-2001). After working as a research scientist at Kosan Biosciences (2001-2002), he started his research group at the Donald Danforth Plant Science Center and moved to the Department of Biology at Washington University in 2008. He has authored more than 160 papers and received a Presidential Early Career Award for Scientists and Engineers (2005), the Phytochemical Society of North America's Arthur Neish Young Investigator Award (2007), and a Fulbright Senior Specialist Award (2012) and was named as Howard Hughes Medical Institute Professor in 2014. Research in the Jez lab seeks to understand how environmental changes re-model biochemical pathways in plants at the molecular, cellular, and organism levels with the aim of engineering these systems to address agricultural and environmental problems. Current work in the lab employs a combination of structural biology, protein chemistry, and plant biology. He also launched the Biotech Explorers Program, which aims to introduce undergraduates to how teams in science can tackle real world problems using cross-disciplinary approaches.

Inhalt

Protein
1. Systems and strategies for plant protein expression
Anthony J. Zmuda, Thomas D. Niehaus
2. Biochemical characterization of plant aromatic aminotransferases
Hiroshi Maeda, Kaan Koper, Andrew Hall, Taichi Takasuka, Shogo Hataya
3. Assaying plant formate-tetrahydrofolate ligase with monoglutamylated and polyglutamylated substrates using a fluorescence-HPLC based method
Sanja Roje, Sompop Saeheng
4. Functional analysis of phosphoethanolamine n-methyltransferases (PMT) in Plants and Parasites: Essential S-Adenosylmethionine (SAM)-dependent Methyltransferase in Choline and Phospholipid Metabolism
Soon Goo Lee
5. An approach to nearest neighbor analysis of pigmented protein complexes using chemical cross-linking in combination with mass spec
Haijun Liu
6. Structural characterization of protein-DNA complexes using SAXS
Chloe Zubieta
7. A fast and cost-effective procedure for reliable measurement of trypsin inihibitor activity in soy and soy products
Hari Krishnan

Metabolite
8. Strategies to study the metabolic origins of specialized plant metabolites
Joshua Widhalm
9. A liquid chromatography-mass spectrometry-based metabolomics strategy to explore plant metabolic diversity
Kazuki Saito, Tetsuya Mori
10. Quantification of plant cardenolides by HPLC, measurement of Na+/K+-ATPase inhibition activity, and characterization of target enzymes
Georg Jander, Georg Petschenka, Tobias Züst, Anurag Agrawal, Amy Hastings
11. Ex vivo metabolomics - a hypothesis-free approach to identify native substrate(s) and product(s) of orphan enzymes
Kirstin Feussner
12. Metabolite fingerprinting: a powerful metabolomics approach for marker identification and functional gene annotation
Ivo Feussner

Environment
13. Controlled environments for cannabis cultivation to support 'omics' research studies and production
Hannah Fleming, Zachary Chamberlain, Jordan J. Zager, B. Markus Lange
14. Standard operating procedures for the comprehensive and reliable analysis of cannabis terpenes
Joshua T. Polito, B. Markus Lange
15. Isolation of novel chemical components and their target proteins in plants under selenium stress
Ryoung Shin, Makoto Muroi, Ju Yeon Moon, Takae Miyazaki, Nobomoto Watanabe
16. Imaging systemic calcium response and its molecular dissection using virus-induced gene silencing
Simon Gilroy
17. Analysis of plant flooding response
Simon Gilroy