Biolistic DNA Delivery in Plants

This volume details protocols for the use of the biolistic DNA delivery method in different plant species. Chapters guide readers through non-protocol chapters that cover relevant topics of interest, a broad overview of the field, exciting modifications of the system, and reliable plant transformation procedures in different plant species. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.

Authoritative and cutting-edge, Biolistic DNA Delivery: Methods and Protocols aims to **provide a comprehensive collection of protocols to intended to be a practical guide for the novice as well as the advanced user in the field of plant genetic transformation.

Includes cutting-edge methods and protocols Provides step-by-step detail essential for reproducible results Contains key notes and implementation advice from the experts

Inhalt

Diter von Wettstein, Professor of Genetics and Master of Translating Science into Applications.- Diter von Wettstein and The Meiotic Program of Pairing and Recombination.- A Short History and Perspectives on Plant Genetic Transformation.- Choice of The Promoter for Tissue and Developmental Stage-specific Gene Expression.- Choice of Explant for Plant Genetic Transformation.- Biolistic Approach for Transient Gene Expression Studies in Plants.- Nano-biolistics: An Emerging Genetic Transformation Approach.- Biolistic Transformation of Japonica Rice Varieties.- Biolistic DNA Delivery in Maize Immature Embryos.- Biolistic DNA Delivery and its Applications in Sorghum bicolor.- Biolistic-mediated Gene Delivery in Sugarcane.- Genetic Transformation of Common Wheat (Triticum aestivum L.) Using Biolistics.- Biolistic DNA Delivery in Turfgrass Embryonic Callus Initiated from Mature Seeds.- Use of Microspore-derived Calli as Explants for Biolistic Transformation of Common Wheat.- Plant Transformation Techniques: Agrobacterium- and Microparticle-mediated Gene Transfer in Cereal Plants.- Proteolistics: A Protein Delivery Method.- Biolistic Delivery of Programmable Nuclease (CRISPR/Cas9) in Bread Wheat.