Biological NMR Part A

Biological NMR, Part A, the latest release in the Methods of Enzymology series, highlights new advances in the field, with this new volume presenting interesting chapters on topics such as Protein methyl labeling, Membrane protein expression - yeast, Protein aromatic labeling, His-tag/Metal contamination, Bicelles, nanodiscs and micelles MP host, PTM - phosphorylation, PTM - lipidation, Screening platform for receptor-ligand discovery

Solution Spectroscopy, Large protein strategies, NUS data collection/analysis, F19 incl. hydration, ODNP - hydration, Reverse micelle - Hydration Solid State Spectroscopy, SS NMR membrane proteins, SS NMR soluble/aggregate proteins, SS DNP - general, SS NMR nucleic acids, Structure determination and computer analysis, and much more.

Autorentext
Dr. A. Joshua Wand teaches at the Department of Biochemistry & Biophysics, Perelman School of Medicine, University of Pennsylvania, USA

Inhalt

1. Selective Methyl Labeling of Proteins: Enabling Structural and Mechanistic Studies As Well As Drug Discovery Applications by Solution-State NMR
   Andrew Proudfoot, Andreas O. Frank, Alexandra Frommlet and Andreas Lingel
   2. Isotopic Labeling of Eukaryotic Membrane Proteins for NMR Studies of Interactions and Dynamics
   Igor Dikiy, Lindsay Clark, Kevin H. Gardner and Daniel M. Rosenbaum
   3. Optimal Isotope Labeling of Aromatic Amino Acid Side Chains for NMR Studies of Protein Dynamics
   Ulrich Weininger
   4. A Quick and Colorful Method to Measure Low-Level Contaminations of Paramagnetic Ni2+ in Protein
   Samples Purified by Immobilized Metal Ion Affinity Chromatography
   Starla D. Glover and Cecilia Tommos
   5. Toolkit for NMR Studies of Methyl-Labeled Proteins
   Paolo Rossi, Yoan R. Monneau, Youlin Xia, Yojiro Ishida and Charalampos G. Kalodimos
   6. Synthetic Biology-Based Solution NMR Studies on Membrane Proteins in Lipid Environments
   Erik Henrich, Frank Löhr, Julija Mezhyrova, Aisha Laguerre, Frank Bernhard and Volker Dötsch
   7. Preparation of Phosphorylated Proteins for NMR Spectroscopy
   Ganesan Senthil Kumar, Rebecca Page and Wolfgang Peti
   8. Synthesis and NMR Characterization of the Prenylated Peptide, a-Factor
   Taysir K. Bader, Todd M. Rappe, Gianlugi Veglia and Mark D. Distefano
   9. The Preparation and Solution NMR Spectroscopy of Human Glycoproteins Is Accessible and Rewarding
   Adam W. Barb, Daniel J. Falconer and Ganesh P. Subedi
   10. Nonuniform Sampling for NMR Spectroscopy
   Scott Robson, Haribabu Arthanari, Sven G. Hyberts and Gerhard Wagner
   11. Exploring Protein Conformational Landscapes Using High-Pressure NMR
   Julien Roche, Catherine A. Royer and Christian Roumestand
   12. CS-ROSETTA
   Santrupti Nerli and Nikolaos G. Sgourakis
   13. Combining Evolutionary Covariance and NMR Data for Protein Structure Determination
   Yuanpeng Janet Huang, Kelly P. Brock, Yojiro Ishida, Gurla V.T. Swapna, Masayori Inouye, Debora S. Marks, Chris Sander and Gaetano T. Montelione
   14. Combining NMR Spectroscopy and Molecular Dynamic Simulations to Solve and Analyse the Structure of Protein-RNA Complexes
   Sebastien Campagne, Miroslav Krepl, Jiri Sponer and Frederic H-T. Allain