Biophysical Tools for Biologists: Volume 89

Informationen zum Autor Professor of Biochemistry and Marine Biology at Northeastern University, promoted 1996. Joined Northeastern faculty in 1987. Previously a faculty member in Dept. of Biochemistry at the University of Mississippi Medical Center, 1983-1987.Principal Investigator in the U.S. Antarctic Program since 1984. Twelve field seasons "on the ice" since 1981. Research conducted at Palmer Station, Antarctica, and McMurdo Station, Antarctica.Research areas: Biochemical, cellular, and physiological adaptation to low and high temperatures. Structure and function of cytoplasmic microtubules and microtubule-dependent motors from cold-adapted Antarctic fishes. Regulation of tubulin and globin gene expression in zebrafish and Antarctic fishes. Role of microtubules in morphogenesis of the zebrafish embryo. Developmental hemapoiesis in zebrafish and Antarctic fishes. UV-induced DNA damage and repair in Antarctic marine organisms. Klappentext Driven in part by the development of genomics, proteomics, and bioinformatics as new disciplines, there has been a tremendous resurgence of interest in physical methods to investigate macromolecular structure and function in the context of living cells. This volume in Methods in Cell Biology is devoted to biophysical techniques in vivo and their applications to cellular biology. The volume covers methods-oriented chapters on fundamental as well as cutting-edge techniques in molecular and cellular biophysics. This book is directed toward the broad audience of cell biologists, biophysicists, pharmacologists, and molecular biologists who employ classical and modern biophysical technologies or wish to expand their expertise to include such approaches. It will also interest the biomedical and biotechnology communities for biophysical characterization of drug formulations prior to FDA approval. Zusammenfassung Covers methods-oriented chapters on fundamental techniques in molecular and cellular biophysics. This book delineates critical steps and potential pitfalls for each method. It is intended for cell biologists! biophysicists! and molecular biologists who employ biophysical technologies or wish to expand their expertise to include such approaches. Inhaltsverzeichnis Section 1. Fluorescence Methods 1) Photoactivation and Photobleaching Techniques for Analysis of Organelle Biogenesis in vivo 2) Analysis of the Dynamics of Living Cells by Fluorescence Correlation Spectroscopy 3) Molecular Sensors Based on Fluorescence Resonance Energy Transfer to Visualize Cellular Dynamics 4) Real-Time Fluorescence of Protein Folding in vivo 5) Microfluidic Glucose Stimulation of Ca+2 Oscillations in Pancreatic Islets Section 2. Microscopic Methods 6) Introduction to Optical Sectioning: Confocal, Deconvolution, and Two-Photon 7) Use of Electron Tomography to Elucidate Sub-Cellular Structure and Function 8) Proteomics of Macromolecular Complexes by Cellular Cryo-Electron Tomography 9) Total Internal Reflectance Microscopy (TIRF) 10) Atomic Force Microscopy of Living Cells 11) Real-Time Kinetics of Gene Activity in Individual Bacteria 12) Measurement of Cytoskeletal Proteins Globally and Locally in vivo 13) Infrared and Raman Microscopy in Cell Biology 14) Imaging Fluorescent Mice in vivo by Confocal Microscopy 15) Nanoscale Imaging of Intracellular Fluorescent Proteins: Breaking the Diffraction Barrier Section 3. Methods at the In Vitro/In Vivo Interface 16) Analysis of Protein Posttranslational Modification by Mass Spectrometry 17) Imaging Mass Spectrometry 18) Wet EM Using Quantum Dots 19) Single Cell Capillary Electrophoresis Section 4. Methods for Diffusion, Viscosity, Force and Displacement 20) Single-Molecule Force Spectroscopy in Living Cells 21) Magnetic Bead Force Applications 22) Measurement of Membrane-Cytoskeleton Adhesion Using Laser Optical Tweezers 2...

Autorentext
Professor of Biochemistry and Marine Biology at Northeastern University, promoted 1996. Joined Northeastern faculty in 1987. Previously a faculty member in Dept. of Biochemistry at the University of Mississippi Medical Center, 1983-1987.Principal Investigator in the U.S. Antarctic Program since 1984. Twelve field seasons "on the ice" since 1981. Research conducted at Palmer Station, Antarctica, and McMurdo Station, Antarctica.Research areas: Biochemical, cellular, and physiological adaptation to low and high temperatures. Structure and function of cytoplasmic microtubules and microtubule-dependent motors from cold-adapted Antarctic fishes. Regulation of tubulin and globin gene expression in zebrafish and Antarctic fishes. Role of microtubules in morphogenesis of the zebrafish embryo. Developmental hemapoiesis in zebrafish and Antarctic fishes. UV-induced DNA damage and repair in Antarctic marine organisms.

Klappentext

Driven in part by the development of genomics, proteomics, and bioinformatics as new disciplines, there has been a tremendous resurgence of interest in physical methods to investigate macromolecular structure and function in the context of living cells. This volume in Methods in Cell Biology is devoted to biophysical techniques in vivo and their applications to cellular biology. The volume covers methods-oriented chapters on fundamental as well as cutting-edge techniques in molecular and cellular biophysics. This book is directed toward the broad audience of cell biologists, biophysicists, pharmacologists, and molecular biologists who employ classical and modern biophysical technologies or wish to expand their expertise to include such approaches. It will also interest the biomedical and biotechnology communities for biophysical characterization of drug formulations prior to FDA approval.

Zusammenfassung
Covers methods-oriented chapters on fundamental techniques in molecular and cellular biophysics. This book delineates critical steps and potential pitfalls for each method. It is intended for cell biologists, biophysicists, and molecular biologists who employ biophysical technologies or wish to expand their expertise to include such approaches.

Inhalt

Section 1. Fluorescence Methods
1) Photoactivation and Photobleaching Techniques for Analysis of Organelle Biogenesis in vivo
2) Analysis of the Dynamics of Living Cells by Fluorescence Correlation Spectroscopy
3) Molecular Sensors Based on Fluorescence Resonance Energy Transfer to Visualize Cellular Dynamics 4) Real-Time Fluorescence of Protein Folding in vivo
5) Microfluidic Glucose Stimulation of Ca+2 Oscillations in Pancreatic Islets

Section 2. Microscopic Methods
6) Introduction to Optical Sectioning: Confocal, Deconvolution, and Two-Photon
7) Use of Electron Tomography to Elucidate Sub-Cellular Structure and Function
8) Proteomics of Macromolecular Complexes by Cellular Cryo-Electron Tomography
9) Total Internal Reflectance Microscopy (TIRF)
10) Atomic Force Microscopy of Living Cells
11) Real-Time Kinetics of Gene Activity in Individual Bacteria
12) Measurement of Cytoskeletal Proteins Globally and Locally in vivo
13) Infrared and Raman Microscopy in Cell Biology
14) Imaging Fluorescent Mice in vivo by Confocal Microscopy
15) Nanoscale Imaging of Intracellular Fluorescent Proteins: Breaking the Diffraction Barrier

Section 3. Methods at the In Vitro/In Vivo Interface
16) Analysis of Protein Posttranslational Modification by Mass Spectrometry
17) Imaging Mass Spectrometry
18) Wet EM Using Quantum Dots
19) Single Cell Capillary Electrophoresis

Section 4. Methods for Diffusion, Viscosity, Force and Displacement
20) Single-Molecule Force Spectroscopy in Living Cells
21) Magnetic Bead Force Applications
22) Measurement of Membrane-Cytoskeleton Adhesion Using Laser Optical Tweezers
23) Cellular Rheological Measurements in vivo
24) Physical Behavior of Cytoskeletal Networks in vitro and in vivo
25) Force Regulation of Microtubule Dynamics in Fission Yeast

Section 5.…