Biotechnology in Agriculture

Twelve mango cultivars grown in the Egypt werediscriminated by their seed SDS-protein and PCR-based molecular markers. For PCR analysis threedifferenttechniquess were used including novel technique.Comparitive analysis were conductied beween the twoold technique, ISSR and RAPD, and the noveltecnique, three primers-based RAPD. The cultivar-specific markers represented 24.46%of the totalmarkers (regardless of type of analysis), 88.89% ofthem were RAPD markers. Most of these markers werescored for the presence of unique bands. Cultivar-specific markers were shown to be useful inconstructing linkage map that involve anypolymorphic gene(s). Dendrogram tree generatedacross SDS-protein, RAPD, ISSR and three promers-based analysis. Constructed dendrograms for results,individually or collectively, revealed thatsimilarity and clustering is dependant on themarker system used.

Autorentext

Dr. Ahmed Mansour is an Egyptian scientist who obtained his
Ph.D. degree in (Plant Signal transduction)He have been invited
and participated in many international scientific meetings and
conferences in molecular genetics around the world. He also has
many scientific publications in the field of Molecular Genetics
and Bioninformatics.

Klappentext

Twelve mango cultivars grown in the Egypt were
discriminated by their seed SDS-protein and PCR-
based molecular markers. For PCR analysis three
different
techniquess were used including novel technique.
Comparitive analysis were conductied beween the two
old technique, ISSR and RAPD, and the novel
tecnique, three primers-based RAPD. The cultivar-
specific markers represented 24.46%of the total
markers (regardless of type of analysis), 88.89% of
them were RAPD markers. Most of these markers were
scored for the presence of unique bands. Cultivar-
specific markers were shown to be useful in
constructing linkage map that involve any
polymorphic gene(s). Dendrogram tree generated
across SDS-protein, RAPD, ISSR and three promers-
based analysis. Constructed dendrograms for results
,individually or collectively, revealed that
similarity and clustering is dependant on the
marker system used.